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Anti-Nebulette Polyclonal Antibody
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Catalog No. TMAB-09328
Anti-Nebulette Polyclonal Antibody is a Rabbit antibody targeting Nebulette. Anti-Nebulette Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Nebulette Polyclonal Antibody
Anti-Nebulette Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-09328
Anti-Nebulette Polyclonal Antibody is a Rabbit antibody targeting Nebulette. Anti-Nebulette Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Antigen Details
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| Description | Anti-Nebulette Polyclonal Antibody is a Rabbit antibody targeting Nebulette. Anti-Nebulette Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse (predicted:Human,Rat) |
| Verified Activity | 1. Tissue/cell: mouse heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-Nebulette Polyclonal Antibody, Unconjugated (TMAB-09328) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 2. Sample: Pancreas (Mouse) Lysate at 40 μg Primary: Anti-Nebulette (TMAB-09328) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 116 kD Observed band size: 116 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasmic. |
| Tissue Specificity | Abundantly expressed in cardiac muscle, but not in skeletal or smooth muscle. Localized to Z-lines in cardiac cells and to dense bodies in nonmuscle cells. Isoform 2 is expressed in non-muscle cells such as in fibroblasts. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Nebulette binds to actin and plays an important role in Z-disk assembly. It is a cardiac homolog of nebulin and its expression is abundant in cardiac muscle. Nebulette defects are assocated with non-familial idiopathic cardiacmyopathy. Nebulin and nebulette are homologous thin-filament associated proteins expressed in skeletal and cardiac muscles, respectively. Nebulette, unlike nebulin, is confined to the Z-disc region of the sarcomere and does not span the whole thin filament length. Nebulette colocalizes with α-actinin in the pre-, nascent, and mature myofibrils. A polymorphism in the actin-binding motif of nebulette is a genetic marker of susceptibility to nonfamilial idiopathic dilated cardiomyopathy (IDC), characterized by a thin-walled heart and systolic dysfunction. |
| Immunogen | KLH conjugated synthetic peptide: human Nebulette |
| Antigen Species | Human |
| Gene Name | NEBL |
| Gene ID | |
| Protein Name | Nebulette |
| Uniprot ID | |
| Function | Binds to actin and plays an important role in the assembly of the Z-disk. Isoform 2 might play a role in the assembly of focal adhesion. |
| Molecular Weight | Theoretical: 116 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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