Anti-NDUFV2 Polyclonal Antibody is a Rabbit antibody targeting NDUFV2. Anti-NDUFV2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF.

IgG

Human,Rat (predicted:Mouse,Chicken,Dog,Pig,Cow,Horse,Rabbit)

1. A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (NDUFV2) polyclonal Antibody, Unconjugated (TMAB-09327) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NDUFV2) Polyclonal Antibody, Unconjugated (TMAB-09327) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human NDUFV2

Human

NDUFV2

NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).