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Anti-NDUFS3 Polyclonal Antibody
Catalog No. TMAB-09322 Copy Product Info
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Anti-NDUFS3 Polyclonal Antibody is a Rabbit antibody targeting NDUFS3. Anti-NDUFS3 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-NDUFS3 Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-09322
Anti-NDUFS3 Polyclonal Antibody is a Rabbit antibody targeting NDUFS3. Anti-NDUFS3 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-NDUFS3 Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-NDUFS3 Polyclonal Antibody is a Rabbit antibody targeting NDUFS3. Anti-NDUFS3 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse (predicted:Human,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit) |
| Verified Activity | 1. Tissue/cell: mouse heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-NDUFS3 Polyclonal Antibody, Unconjugated (TMAB-09322) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 2. Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NDUFS3) Polyclonal Antibody, Unconjugated (TMAB-09322) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Mitochondrion inner membrane. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The multisubunit NADH:ubiquinone oxidoreductase is the first enzyme complex in the electron transport chain of mitochondria. By use of chaotropic agents, complex I can be fragmented into 3 different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. The IP fraction contains NDUFS1, NDUFS2, NDUFS3, NDUFS4, NDUFS5, NDUFS6, and NDUFA5. |
| Immunogen | KLH conjugated synthetic peptide: human NDUFS3 |
| Antigen Species | Human |
| Gene Name | NDUFS3 |
| Gene ID | |
| Protein Name | NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial |
| Uniprot ID |
| Molecular Weight | Theoretical: 30 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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