Anti-NDUFS3 Antibody (4H392) is a Rabbit antibody targeting NDUFS3. Anti-NDUFS3 Antibody (4H392) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,IP.

IgG

4H392

Human

1. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFS3 Monoclonal Antibody, Unconjugated (TMAB-09323) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFS3 Monoclonal Antibody, Unconjugated (TMAB-09323) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFS3 Monoclonal Antibody, Unconjugated (TMAB-09323) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFS3 Monoclonal Antibody, Unconjugated (TMAB-09323) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. 25 μg total protein per lane of various lysates (see on figure) probed with NDUFS3 monoclonal antibody, unconjugated (TMAB-09323) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200; IP: 1:20-50

Monoclonal

A synthesized peptide: human NDUFS3

Human

NDUFS3

NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial