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Anti-NDST4 Polyclonal Antibody
Catalog No. TMAB-09303 Copy Product Info
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Anti-NDST4 Polyclonal Antibody is a Rabbit antibody targeting NDST4. Anti-NDST4 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-NDST4 Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-09303
Anti-NDST4 Polyclonal Antibody is a Rabbit antibody targeting NDST4. Anti-NDST4 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-NDST4 Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-NDST4 Polyclonal Antibody is a Rabbit antibody targeting NDST4. Anti-NDST4 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse (predicted:Human,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NDST4) Polyclonal Antibody, Unconjugated (TMAB-09303) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Golgi apparatus membrane; Single-pass type II membrane protein |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The N-deacetylation and N-sulfation of N-acetylglucosamine residues in heparan sulfate and heparin initiate a set of biochemical reactions, which lead to the synthesis of oligosaccharide sequences that have specific ligand binding properties (1). These reactions are catalyzed by the monomeric enzymes GlcNAc Ndeacetylase/N-sulfotransferases (NDSTs), which have two catalytic activities (1). Multiple NDST isozymes have been identified, each having unique tissue distribution and enzymatic properties (2). Phylogenetic data suggests that NDST1-4 evolved from a common ancestral gene, which diverged to give rise to two subtypes, NDST1/2 and NDST3/4 (2). NDST1, which maps to human chromosome 5q32-q33.1, shares the most homology with NDST2, which maps to human chromosome 10q22 (3,4). The least conserved amino acids between these two enzymes are found in the N-terminus/putative transmembrane regions (3). The human NDST3 and NDST4 genes are closely linked on chromosome 4, mapping to chromosome 4q25-26 and 4q26-27, respectively (2,5,6). RT-PCR analysis of various mouse tissues reveals a restricted pattern of NDST3 and NDST4 mRNA expression when compared with that of NDST1 and NDST2, which are abundantly and ubiquitously expressed (2). |
| Immunogen | KLH conjugated synthetic peptide: human NDST4 |
| Antigen Species | Human |
| Gene Name | NDST4 |
| Gene ID | |
| Protein Name | Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 4 |
| Uniprot ID | |
| Function | Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Has low deacetylase activity but high sulfotransferase activity |
| Molecular Weight | Theoretical: 101 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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