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Anti-NCKAP1 Polyclonal Antibody

Catalog No. TMAB-09279 Copy Product Info
🥰Excellent
Anti-NCKAP1 Polyclonal Antibody is a Rabbit antibody targeting NCKAP1. Anti-NCKAP1 Polyclonal Antibody can be used in WB.

Anti-NCKAP1 Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-09279

Anti-NCKAP1 Polyclonal Antibody is a Rabbit antibody targeting NCKAP1. Anti-NCKAP1 Polyclonal Antibody can be used in WB.

Anti-NCKAP1 Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2227-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5287-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-NCKAP1 Polyclonal Antibody is a Rabbit antibody targeting NCKAP1. Anti-NCKAP1 Polyclonal Antibody can be used in WB.
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Horse,GuineaPig)
Verified Activity
1. Sample: Skeletal muscle (Mouse) Lysate at 40 μg
Primary: Anti-NCKAP1 (TMAB-09279) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 129 kD
Observed band size: 129 kD
2. Sample:
Lane 1: Human HepG2 cell lysates
Lane 2: Human HeLa cell lysates
Lane 3: Human 293T cell lysates
Primary: Anti-NCKAP1 (TMAB-09279) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 129 kDa
Observed band size: 135 kDa
Application
WB
Recommended Dose
WB: 1:500-2000
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane. Cell projection
Tissue SpecificityExpressed in all tissues examined except peripheral blood leukocytes, with highest expression in brain, heart, and skeletal muscle. Expressed in cells of various brain regions including Purkinje cells and dentate nucleus of the cerebellum, CA4 region and dentate gyrus of the hippocampus, and in frontal gray and white matter
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundContributes to small GTPase binding activity. Involved in Rac protein signal transduction; positive regulation of Arp2/3 complex-mediated actin nucleation; and positive regulation of lamellipodium assembly. Located in extracellular exosome and focal adhesion. Part of SCAR complex. [provided by Alliance of Genome Resources, Apr 2022]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human NCKAP1
Antigen Species
Human
Gene Name
NCKAP1
Gene ID
10787
Protein Name
Nck-associated protein 1
Uniprot ID
Q9Y2A7
Function
Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes.
Chemical Properties
Molecular WeightTheoretical: 129 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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