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Anti-NCAM1 Polyclonal Antibody is a Rabbit antibody targeting NCAM1. Anti-NCAM1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $221 | 7-10 days | |
100 μL | $372 | 7-10 days | |
200 μL | $529 | 7-10 days |
Description | Anti-NCAM1 Polyclonal Antibody is a Rabbit antibody targeting NCAM1. Anti-NCAM1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Ig Type | IgG |
Reactivity | Human,Rat (predicted:Mouse,Chicken,Dog,Pig,Cow,Horse,Rabbit) |
Verified Activity | 1. Protein: Hela (human) cell lysates at 40 μg; Primary: rabbit Anti-CD56 (TMAB-01204) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit Igg (secondary antibody) at 1: 5000; Predicted band size:92 kDa Observed band size:125 kDa 2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-NCAM1 Polyclonal Antibody, Unconjugated (TMAB-01204) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. |
Application | WB,IHC-P,IHC-Fr,IF |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Isoform 1, 2,: Cell membrane; Single-pass type I membrane protein. Isoform 3, 4: Cell membrane; Lipid-anchor, GPI-anchor. Isoform 5, 6: Secreted. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011] |
Immunogen | KLH conjugated synthetic peptide: human CD56/NCAM1 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | ECM Proteins,Growth Cone,Neural Signal Transduction,Human Lineage Negative |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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