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Anti-NCAM1 Polyclonal Antibody

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Catalog No. TMAB-01204

Anti-NCAM1 Polyclonal Antibody is a Rabbit antibody targeting NCAM1. Anti-NCAM1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-NCAM1 Polyclonal Antibody

Anti-NCAM1 Polyclonal Antibody

😃Good
Catalog No. TMAB-01204
Anti-NCAM1 Polyclonal Antibody is a Rabbit antibody targeting NCAM1. Anti-NCAM1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$221 7-10 days
100 μL$372 7-10 days
200 μL$529 7-10 days
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Product Introduction

Bioactivity
Description
Anti-NCAM1 Polyclonal Antibody is a Rabbit antibody targeting NCAM1. Anti-NCAM1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Rat (predicted:Mouse,Chicken,Dog,Pig,Cow,Horse,Rabbit)
Verified Activity
1. Protein: Hela (human) cell lysates at 40 μg;
Primary: rabbit Anti-CD56 (TMAB-01204) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit Igg (secondary antibody) at 1: 5000;
Predicted band size:92 kDa
Observed band size:125 kDa
2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-NCAM1 Polyclonal Antibody, Unconjugated (TMAB-01204) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationIsoform 1, 2,: Cell membrane; Single-pass type I membrane protein. Isoform 3, 4: Cell membrane; Lipid-anchor, GPI-anchor. Isoform 5, 6: Secreted.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CD56/NCAM1
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
ECM Proteins,Growth Cone,Neural Signal Transduction,Human Lineage Negative
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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