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Anti-NCAM Antibody (2S376) is a Mouse antibody targeting NCAM. Anti-NCAM Antibody (2S376) can be used in IHC-P,IHC-Fr,IF,FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 100 μg | $353 | 7-10 days | 7-10 days |
| Description | Anti-NCAM Antibody (2S376) is a Mouse antibody targeting NCAM. Anti-NCAM Antibody (2S376) can be used in IHC-P,IHC-Fr,IF,FCM. |
| Ig Type | Mouse IgG2a, k |
| Clone | 2S376 |
| Reactivity | Human |
| Verified Activity | 1. scatter diagram showing peripheral blood lymphocytes stained with CD56. The cells were incubated with the antibody (TMAB-09267) for 30 min at 22°C. The secondary antibody used for 40 min at room temperature. Acquisition of >10,000 events was performed. 2. Paraformaldehyde-fixed, paraffin embedded Human Prostate Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with human CD56 (NCAM) Monoclonal Antibody, Unconjugated (TMAB-09267) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with human CD56 (NCAM) Monoclonal Antibody, Unconjugated (TMAB-09267) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with human CD56 (NCAM) Monoclonal Antibody, Unconjugated (TMAB-09267) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with human CD56 (NCAM) Monoclonal Antibody, Unconjugated (TMAB-09267) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with human CD56 (NCAM) Monoclonal Antibody, Unconjugated (TMAB-09267) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Left Parietal Lobe; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with human CD56 (NCAM) Monoclonal Antibody, Unconjugated (TMAB-09267) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Isoform 1, 2,: Cell membrane; Single-pass type I membrane protein. Isoform 3, 4: Cell membrane; Lipid-anchor, GPI-anchor. Isoform 5, 6: Secreted. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4). |
| Research Background | This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011] |
| Immunogen | KLH conjugated synthetic peptide: human CD56 |
| Antigen Species | Human |
| Gene Name | NCAM1 |
| Gene ID | |
| Protein Name | Neural cell adhesion molecule 1 |
| Uniprot ID | |
| Function | This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
Subcellular Location : Isoform 1, 2,: Cell membrane; Single-pass type I membrane protein. Isoform 3, 4: Cell membrane; Lipid-anchor, GPI-anchor. Isoform 5, 6: Secreted. |
| Molecular Weight | Theoretical: 92 kDa. Actual: 140 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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