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Anti-NCAM Antibody (2S376)

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Catalog No. TMAB-09267

Anti-NCAM Antibody (2S376) is a Mouse antibody targeting NCAM. Anti-NCAM Antibody (2S376) can be used in IHC-P,IHC-Fr,IF,FCM.

Anti-NCAM Antibody (2S376)

Anti-NCAM Antibody (2S376)

Copy Product Info
😃Good
Catalog No. TMAB-09267
Anti-NCAM Antibody (2S376) is a Mouse antibody targeting NCAM. Anti-NCAM Antibody (2S376) can be used in IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
100 μg$3537-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-NCAM Antibody (2S376) is a Mouse antibody targeting NCAM. Anti-NCAM Antibody (2S376) can be used in IHC-P,IHC-Fr,IF,FCM.
Ig Type
Mouse IgG2a, k
Clone
2S376
Reactivity
Human
Verified Activity
1. scatter diagram showing peripheral blood lymphocytes stained with CD56. The cells were incubated with the antibody (TMAB-09267) for 30 min at 22°C. The secondary antibody used for 40 min at room temperature. Acquisition of >10,000 events was performed.
2. Paraformaldehyde-fixed, paraffin embedded Human Prostate Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with human CD56 (NCAM) Monoclonal Antibody, Unconjugated (TMAB-09267) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with human CD56 (NCAM) Monoclonal Antibody, Unconjugated (TMAB-09267) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with human CD56 (NCAM) Monoclonal Antibody, Unconjugated (TMAB-09267) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with human CD56 (NCAM) Monoclonal Antibody, Unconjugated (TMAB-09267) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with human CD56 (NCAM) Monoclonal Antibody, Unconjugated (TMAB-09267) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Left Parietal Lobe; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with human CD56 (NCAM) Monoclonal Antibody, Unconjugated (TMAB-09267) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationIsoform 1, 2,: Cell membrane; Single-pass type I membrane protein. Isoform 3, 4: Cell membrane; Lipid-anchor, GPI-anchor. Isoform 5, 6: Secreted.
ConstructionHybridoma Monoclonal Antibody
PurificationProtein G purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4).
Research BackgroundThis gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CD56
Antigen Species
Human
Gene Name
NCAM1
Gene ID
Protein Name
Neural cell adhesion molecule 1
Uniprot ID
Function
This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
Subcellular Location : Isoform 1, 2,: Cell membrane; Single-pass type I membrane protein. Isoform 3, 4: Cell membrane; Lipid-anchor, GPI-anchor. Isoform 5, 6: Secreted.
Chemical Properties
Molecular WeightTheoretical: 92 kDa. Actual: 140 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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