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Anti-NAPSIN A Antibody (6H377)
Catalog No. TMAB-09239 Copy Product Info
🥰Excellent
Anti-NAPSIN A Antibody (6H377) is a Mouse antibody targeting NAPSIN A. Anti-NAPSIN A Antibody (6H377) can be used in IHC-P,IF,IHC-Fr.
Anti-NAPSIN A Antibody (6H377)
Copy Product Info🥰Excellent
Catalog No. TMAB-09239
Anti-NAPSIN A Antibody (6H377) is a Mouse antibody targeting NAPSIN A. Anti-NAPSIN A Antibody (6H377) can be used in IHC-P,IF,IHC-Fr.
Anti-NAPSIN A Antibody
(6H377)
(6H377)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
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| Description | Anti-NAPSIN A Antibody (6H377) is a Mouse antibody targeting NAPSIN A. Anti-NAPSIN A Antibody (6H377) can be used in IHC-P,IF,IHC-Fr. |
| Ig Type | IgG1, Kappa |
| Clone | 6H377 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (NAPSIN A) Monoclonal Antibody, Unconjugated (TMAB-09239) at 1:100overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (NAPSIN A) Monoclonal Antibody, Unconjugated (TMAB-09239) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (human lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (NAPSIN A) Monoclonal Antibody, Unconjugated (TMAB-09239) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (NAPSIN A) Monoclonal Antibody, Unconjugated (TMAB-09239) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (NAPSIN A) Monoclonal Antibody, Unconjugated (TMAB-09239) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IF: 1:100-500; IHC-Fr: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Secreted (Potential). |
| Tissue Specificity | Expressed predominantly in adult lung (type II pneumocytes) and kidney and in fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Proclin300. |
| Concentration | 1mg/ml |
| Research Background | Napsin A is an aspartic proteinase that belongs to the peptidase A1 family and plays a role in pneumocyte surfactant processing. It is a 420-amino acid polypeptide consisting of a 24-residue signal peptide, a 40-amino acid propart, the mature enzyme of 336 amino acids, and a C-terminal extension of 18 residues. The mature Napsin A protein contains 3 predicted disulfide bonds, 3 potential N-linked oligosaccharide attachment sites, an RGD motif, a recognition motif for integrin binding, in the C terminus, immediately before a 4-amino acid insert that is unique to aspartic proteinases. Highest levels of Napsin A have been detected in adult lung (type II pneumocytes), fetal lung, and kidney tissues. Napsin A is also expressed at lower levels in adult spleen and at very low levels in peripheral blood leukocytes. Human napsin A shares 72.6% sequence identity with the mouse homolog. |
| Immunogen | KLH conjugated synthetic peptide: mouse NAPSIN A |
| Antigen Species | Mouse |
| Gene Name | NAPSA |
| Gene ID | |
| Protein Name | Napsin-A |
| Function | May be involved in processing of pneumocyte surfactant precursors. |
| Molecular Weight | Theoretical: 39 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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