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Anti-NAPSIN A Antibody (6H195) is a Rabbit antibody targeting NAPSIN A. Anti-NAPSIN A Antibody (6H195) can be used in WB,IHC-P,IHC-Fr,IP,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $150 | 7-10 days | 7-10 days | |
| 50 μL | $262 | 7-10 days | 7-10 days | |
| 100 μL | $473 | 7-10 days | 7-10 days |
| Description | Anti-NAPSIN A Antibody (6H195) is a Rabbit antibody targeting NAPSIN A. Anti-NAPSIN A Antibody (6H195) can be used in WB,IHC-P,IHC-Fr,IP,IF. |
| Ig Type | IgG |
| Clone | 6H195 |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of Human lung adenocarcinoma lysate. Using NAPSIN A (TMAB-09240) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 2. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NAPSIN A Monoclonal Antibody, Unconjugated (TMAB-09240) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NAPSIN A Monoclonal Antibody, Unconjugated (TMAB-09240) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NAPSIN A Monoclonal Antibody, Unconjugated (TMAB-09240) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 5. (Negative control) paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NAPSIN A Monoclonal Antibody, Unconjugated (TMAB-09240) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IP: 1:20-50; IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted (Potential). |
| Tissue Specificity | Expressed predominantly in adult lung (type II pneumocytes) and kidney and in fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| Research Background | May be involved in processing of pneumocyte surfactant precursors. |
| Immunogen | A synthesized peptide: human Napsin A |
| Antigen Species | Human |
| Gene Name | NAPSA |
| Gene ID | |
| Protein Name | Napsin-A |
| Uniprot ID | |
| Function | May be involved in processing of pneumocyte surfactant precursors. |
| Molecular Weight | Theoretical: 45 kDa. Actual: 45 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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