Anti-NALP2 Polyclonal Antibody is a Rabbit antibody targeting NALP2. Anti-NALP2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Horse,Rabbit)

1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-NALP2/PAN1/PYPAF2 Polyclonal Antibody, Unconjugated (TMAB-09214) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample:
Thymus (Mouse) Lysate at 40 μg
A431 (Human) Cell Lysate at 30 μg
Molt-4 (Human) Cell Lysate at 30 μg
Primary: Anti-NALP2 (TMAB-09214) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 117 kD
Observed band size: 117 kD
3. Sample:
Lane 1: Mouse Thymus tissue lysates
Lane 2: Mouse Ovary tissue lysates
Lane 3: Rat Placenta tissue lysates
Lane 4: Human MCF-7 cell lysates
Primary: Anti-NALP2 (TMAB-09214) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 117 kDa
Observed band size: 120 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human NACHT, LRR and PYD domains-containing protein 2

Human

NLRP2

NACHT, LRR and PYD domains-containing protein 2

Suppresses TNF- and CD40-induced NFKB1 activity at the level of the IKK complex, by inhibiting NFKBIA degradation induced by TNF. When associated with PYCARD, activates CASP1, leading to the secretion of mature proinflammatory cytokine IL1B. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases.