Shopping Cart
  • Remove All
  • TargetMol
    Your shopping cart is currently empty

Anti-NAA10/ARD1 Antibody (8M884)

😃Good
Catalog No. TMAY-02716
Alias TE2, OGDNS, NATD, NAA10, N(α)-acetyltransferase 10, NatA catalytic subunit, N(alpha)-acetyltransferase 10, NatA catalytic subunit, MCOPS1, DXS707, ARD1P, ARD1A, ARD1

Anti-NAA10/ARD1 Antibody (8M884) is a Mouse antibody targeting NAA10/ARD1. Anti-NAA10/ARD1 Antibody (8M884) can be used in WB,IP.

Anti-NAA10/ARD1 Antibody (8M884)

Anti-NAA10/ARD1 Antibody (8M884)

😃Good
Catalog No. TMAY-02716Alias TE2, OGDNS, NATD, NAA10, N(α)-acetyltransferase 10, NatA catalytic subunit, N(alpha)-acetyltransferase 10, NatA catalytic subunit, MCOPS1, DXS707, ARD1P, ARD1A, ARD1
Anti-NAA10/ARD1 Antibody (8M884) is a Mouse antibody targeting NAA10/ARD1. Anti-NAA10/ARD1 Antibody (8M884) can be used in WB,IP.
Pack SizePriceAvailabilityQuantity
100 μL$198 7-10 days
Bulk & Custom
Add to Cart
Questions
View More
Contact us for more batch information
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Product Introduction

Bioactivity
Description
Anti-NAA10/ARD1 Antibody (8M884) is a Mouse antibody targeting NAA10/ARD1. Anti-NAA10/ARD1 Antibody (8M884) can be used in WB,IP.
AliasTE2, OGDNS, NATD, NAA10, N(α)-acetyltransferase 10, NatA catalytic subunit, N(alpha)-acetyltransferase 10, NatA catalytic subunit, MCOPS1, DXS707, ARD1P, ARD1A, ARD1
Ig Type
Monoclonal Mouse IgG2b
Clone
8M884
Reactivity
Human
Verified Activity
1. ARD1A was immunoprecipitated using:

-Lane A:0.5 mg Hela Whole Cell Lysate

-0.5 µL anti-ARD1A mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-ARD1A mouse monoclonal antibody, at 1:500 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 33 kDa.

-Observed band size: 26 kDa.

2. Anti-ARD1A mouse monoclonal antibody at 1:500 dilution.

-Lane A: Hela Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:26 kDa.

-Observed band size:33 kDa
Application
WB,IP
Recommended Dose
WB: 1:500-1:1000; IP: 0.2-1 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ARD1 (rh ARD1; TMPY-03339; P41227; Met1-Ser235). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundARD1 is a member of the 2-kDa ARF protein family. It is a multifunctional protein. ARD1 has an 18-kDa ADP-ribosylation factor (ARF) domain at the C-terminus (amino acids 43-574), and a 46-kDa N-terminal domain (amino acids 1-42). The C-terminal region of ARD1 may be involved in the formation of both ARD1-ARD1 and ARD1-NAT1 complexes. ARD1 and NAT1 genes are required for the expression of an N-terminal protein acetyltransferase. This activity is required for full repression of the silent mating-type locus HML, for sporulation, and for entry into G. Recombinant ARD1 (amino acids 1-574) or its RING finger domain (amino acids 1-11) produced polyubiquitylated proteins when incubated in vitro with a mammalian E1, an E2 enzyme, ATP, and ubiquitin.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human ARD1 protein (TMPY-03339)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

Sci Citations

Calculator

  • Molarity Calculator
  • Dilution Calculator
  • Reconstitution Calculator
  • Molecular Weight Calculator

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: buy Anti-NAA10/ARD1 Antibody (8M884) | purchase Anti-NAA10/ARD1 Antibody (8M884) | Anti-NAA10/ARD1 Antibody (8M884) cost | order Anti-NAA10/ARD1 Antibody (8M884)