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Anti-NAA10/ARD1 Antibody (8M884) (Synonyms: TE2, OGDNS, NATD, NAA10, N(α)-acetyltransferase 10, NatA catalyt...)

Catalog No. TMAY-02716 Copy Product Info
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Anti-NAA10/ARD1 Antibody (8M884) is a Mouse antibody targeting NAA10/ARD1. Anti-NAA10/ARD1 Antibody (8M884) can be used in IP,WB.

Anti-NAA10/ARD1 Antibody (8M884)

Copy Product Info
🥰Excellent
Catalog No. TMAY-02716
Synonyms TE2, OGDNS, NATD, NAA10, N(α)-acetyltransferase 10, NatA catalytic subunit, N(alpha)-acetyltransferase 10, NatA catalytic subunit, MCOPS1, DXS707, ARD1P, ARD1A, ARD1

Anti-NAA10/ARD1 Antibody (8M884) is a Mouse antibody targeting NAA10/ARD1. Anti-NAA10/ARD1 Antibody (8M884) can be used in IP,WB.

Anti-NAA10/ARD1 Antibody
(8M884)
Pack SizePriceUSA StockGlobal StockQuantity
100 μL$1987-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-NAA10/ARD1 Antibody (8M884) is a Mouse antibody targeting NAA10/ARD1. Anti-NAA10/ARD1 Antibody (8M884) can be used in IP,WB.
SynonymsTE2, OGDNS, NATD, NAA10, N(α)-acetyltransferase 10, NatA catalytic subunit, N(alpha)-acetyltransferase 10, NatA catalytic subunit, MCOPS1, DXS707, ARD1P, ARD1A, ARD1
Ig Type
Mouse IgG2b
Clone
8M884
Reactivity
Human
Specificity
Human NAA10/ARD1
Verified Activity
1. ARD1A was immunoprecipitated using:
-Lane A:0.5 mg Hela Whole Cell Lysate
-0.5 µL anti-ARD1A mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-ARD1A mouse monoclonal antibody, at 1:500 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 33 kDa.
-Observed band size: 26 kDa.
2. Anti-ARD1A mouse monoclonal antibody at 1:500 dilution.
-Lane A: Hela Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:26 kDa.
-Observed band size:33 kDa
Application
Recommended Dose
WB: 1:500-1:1000; IP: 0.2-1 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ARD1 (rh ARD1; TMPY-03339; P41227; Met1-Ser235). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundARD1 is a member of the 2-kDa ARF protein family. It is a multifunctional protein. ARD1 has an 18-kDa ADP-ribosylation factor (ARF) domain at the C-terminus (amino acids 43-574), and a 46-kDa N-terminal domain (amino acids 1-42). The C-terminal region of ARD1 may be involved in the formation of both ARD1-ARD1 and ARD1-NAT1 complexes. ARD1 and NAT1 genes are required for the expression of an N-terminal protein acetyltransferase. This activity is required for full repression of the silent mating-type locus HML, for sporulation, and for entry into G. Recombinant ARD1 (amino acids 1-574) or its RING finger domain (amino acids 1-11) produced polyubiquitylated proteins when incubated in vitro with a mammalian E1, an E2 enzyme, ATP, and ubiquitin.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: Human ARD1 protein (TMPY-03339)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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