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Anti-NAA10/ARD1 Antibody (6X451)

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Catalog No. TMAY-02715
Alias TE2, OGDNS, NATD, NAA10, N(α)-acetyltransferase 10, NatA catalytic subunit, N(alpha)-acetyltransferase 10, NatA catalytic subunit, MCOPS1, DXS707, ARD1P, ARD1A, ARD1

Anti-NAA10/ARD1 Antibody (6X451) is a Mouse antibody targeting NAA10/ARD1. Anti-NAA10/ARD1 Antibody (6X451) can be used in FCM,ICC/IF.

Anti-NAA10/ARD1 Antibody (6X451)

Anti-NAA10/ARD1 Antibody (6X451)

😃Good
Catalog No. TMAY-02715Alias TE2, OGDNS, NATD, NAA10, N(α)-acetyltransferase 10, NatA catalytic subunit, N(alpha)-acetyltransferase 10, NatA catalytic subunit, MCOPS1, DXS707, ARD1P, ARD1A, ARD1
Anti-NAA10/ARD1 Antibody (6X451) is a Mouse antibody targeting NAA10/ARD1. Anti-NAA10/ARD1 Antibody (6X451) can be used in FCM,ICC/IF.
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100 μL188 € 7-10 days
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Product Introduction

Bioactivity
Description
Anti-NAA10/ARD1 Antibody (6X451) is a Mouse antibody targeting NAA10/ARD1. Anti-NAA10/ARD1 Antibody (6X451) can be used in FCM,ICC/IF.
AliasTE2, OGDNS, NATD, NAA10, N(α)-acetyltransferase 10, NatA catalytic subunit, N(alpha)-acetyltransferase 10, NatA catalytic subunit, MCOPS1, DXS707, ARD1P, ARD1A, ARD1
Ig Type
Monoclonal Mouse IgG2a
Clone
6X451
Reactivity
Human
Verified Activity
1. Immunofluorescence staining of Human ARD1A in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human ARD1A monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.

2. Flow cytometric analysis of Human ARD1A expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human ARD1A, then a PE-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Application
FCM,ICC/IF
Recommended Dose
ICC-IF: 1:20-1:100; FCM: 1:25-1:100
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ARD1 (rh ARD1; TMPY-03339; P41227; Met1-Ser235). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundARD1 is a member of the 2-kDa ARF protein family. It is a multifunctional protein. ARD1 has an 18-kDa ADP-ribosylation factor (ARF) domain at the C-terminus (amino acids 43-574), and a 46-kDa N-terminal domain (amino acids 1-42). The C-terminal region of ARD1 may be involved in the formation of both ARD1-ARD1 and ARD1-NAT1 complexes. ARD1 and NAT1 genes are required for the expression of an N-terminal protein acetyltransferase. This activity is required for full repression of the silent mating-type locus HML, for sporulation, and for entry into G. Recombinant ARD1 (amino acids 1-574) or its RING finger domain (amino acids 1-11) produced polyubiquitylated proteins when incubated in vitro with a mammalian E1, an E2 enzyme, ATP, and ubiquitin.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human ARD1 protein (TMPY-03339)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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