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Anti-NAA10/ARD1 Antibody (6X451)
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Catalog No. TMAY-02715
Alias TE2, OGDNS, NATD, NAA10, N(α)-acetyltransferase 10, NatA catalytic subunit, N(alpha)-acetyltransferase 10, NatA catalytic subunit, MCOPS1, DXS707, ARD1P, ARD1A, ARD1
Anti-NAA10/ARD1 Antibody (6X451) is a Mouse antibody targeting NAA10/ARD1. Anti-NAA10/ARD1 Antibody (6X451) can be used in FCM,ICC/IF.
Anti-NAA10/ARD1 Antibody (6X451)
Anti-NAA10/ARD1 Antibody (6X451)
😃Good
Catalog No. TMAY-02715Alias TE2, OGDNS, NATD, NAA10, N(α)-acetyltransferase 10, NatA catalytic subunit, N(alpha)-acetyltransferase 10, NatA catalytic subunit, MCOPS1, DXS707, ARD1P, ARD1A, ARD1
Anti-NAA10/ARD1 Antibody (6X451) is a Mouse antibody targeting NAA10/ARD1. Anti-NAA10/ARD1 Antibody (6X451) can be used in FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $198 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: FCM, ICC/IF Reactivity: Human |
| Alias | TE2, OGDNS, NATD, NAA10, N(α)-acetyltransferase 10, NatA catalytic subunit, N(alpha)-acetyltransferase 10, NatA catalytic subunit, MCOPS1, DXS707, ARD1P, ARD1A, ARD1 |
| Ig Type | Mouse IgG2a |
| Clone | 6X451 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Human ARD1A in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human ARD1A monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus. 2. Flow cytometric analysis of Human ARD1A expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human ARD1A, then a PE-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | FCM,ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ARD1 (rh ARD1; TMPY-03339; P41227; Met1-Ser235). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | ARD1 is a member of the 2-kDa ARF protein family. It is a multifunctional protein. ARD1 has an 18-kDa ADP-ribosylation factor (ARF) domain at the C-terminus (amino acids 43-574), and a 46-kDa N-terminal domain (amino acids 1-42). The C-terminal region of ARD1 may be involved in the formation of both ARD1-ARD1 and ARD1-NAT1 complexes. ARD1 and NAT1 genes are required for the expression of an N-terminal protein acetyltransferase. This activity is required for full repression of the silent mating-type locus HML, for sporulation, and for entry into G. Recombinant ARD1 (amino acids 1-574) or its RING finger domain (amino acids 1-11) produced polyubiquitylated proteins when incubated in vitro with a mammalian E1, an E2 enzyme, ATP, and ubiquitin. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human ARD1 protein (TMPY-03339) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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