Anti-N WASP Antibody (9A179) is a Rabbit antibody targeting N WASP. Anti-N WASP Antibody (9A179) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.

IgG

9A179

Human,Mouse,Rat

1. 25 μg total protein per lane of various lysates (see on figure) probed with N WASP monoclonal antibody, unconjugated (TMAB-09202) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NWASP Monoclonal Antibody, Unconjugated (TMAB-09202) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NWASP Monoclonal Antibody, Unconjugated (TMAB-09202) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NWASP Monoclonal Antibody, Unconjugated (TMAB-09202) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500; FCM: 1:50-100

Monoclonal

A synthesized peptide: human N WASP

Human

WASL

Neural Wiskott-Aldrich syndrome protein

Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression.