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Anti-myosin light chain 3 Antibody (3M242)
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Catalog No. TMAB-09182
Anti-myosin light chain 3 Antibody (3M242) is a Rabbit antibody targeting myosin light chain 3. Anti-myosin light chain 3 Antibody (3M242) can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Anti-myosin light chain 3 Antibody (3M242)
Anti-myosin light chain 3 Antibody (3M242)
Copy Product Info😃Good
Catalog No. TMAB-09182
Anti-myosin light chain 3 Antibody (3M242) is a Rabbit antibody targeting myosin light chain 3. Anti-myosin light chain 3 Antibody (3M242) can be used in WB,IHC-P,IHC-Fr,IF,FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $148 | 7-10 days | 7-10 days | |
| 50 μL | $263 | 7-10 days | 7-10 days | |
| 100 μL | $470 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-myosin light chain 3 Antibody (3M242) is a Rabbit antibody targeting myosin light chain 3. Anti-myosin light chain 3 Antibody (3M242) can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
| Ig Type | IgG |
| Clone | 3M242 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Cell line: U-2 OS Fixative: 4% Paraformaldehyde Permeabilization: 90% methanol Primary ab dilution: 1:100 Secondary ab: Goat anti Rabbit IgG Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for TMAB-09182 2. Tissue: Rat cardiac muscle Section type: Formalin fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit (Rabbit) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for TMAB-09182 3. Tissue: Mouse skeletal muscle Section type: Formalin fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit (Rabbit) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for TMAB-09182 4. Tissue: Human skeletal muscle Section type: Formalin fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit (Rabbit) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for TMAB-09182 5. Tissue: Mouse cardiac muscle Section type: Formalin fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit (Rabbit) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for TMAB-09182 6. Tissue: Rat skeletal muscle Section type: Formalin fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit (Rabbit) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for TMAB-09182 7. Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1: 2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: Mouse heart, Rat heart Protein loading quantity: 20 μg Exposure time: 10 s Predicted MW: 22 kDa Observed MW: 22 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways which increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. Human myosin light chain has clinical application as a cardiac marker. |
| Gene Name | MYL3 |
| Gene ID | |
| Protein Name | Myosin light chain 3 |
| Function | Regulatory light chain of myosin. Does not bind calcium. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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