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Anti-MYO16 Polyclonal Antibody

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Catalog No. TMAB-09164

Anti-MYO16 Polyclonal Antibody is a Rabbit antibody targeting MYO16. Anti-MYO16 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-MYO16 Polyclonal Antibody

Anti-MYO16 Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-09164
Anti-MYO16 Polyclonal Antibody is a Rabbit antibody targeting MYO16. Anti-MYO16 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2217-10 days7-10 days
100 μL$3747-10 days7-10 days
200 μL$5277-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-MYO16 Polyclonal Antibody is a Rabbit antibody targeting MYO16. Anti-MYO16 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Mouse,Rat (predicted:Human,Chicken,Zebrafish,Fruit Fly)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MYO16) Polyclonal Antibody, Unconjugated (TMAB-09164) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Sample:
Cerebrum (Mouse) Lysate at 40 μg
Cerebrum (Rat) Lysate at 40 μg
Primary: Anti-MYO16 (TMAB-09164) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 206 kD
Observed band size: 123 kD
3. Sample:
Brain (Mouse) Lysate at 40 μg
Brain (Rat) Lysate at 40 μg
Primary: Anti-MYO16 (TMAB-09164) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 206 kD
Observed band size: 123 kD
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Found in puncta in soma and processes of astrocytes and dissociated cerebellar cells with the morphology of migrating granule cells.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundMyosin XVI is a 1,858 amino acid protein that localizes to the cytoplasm and contains one IQ domain, two myosin head-like domains and seven ANK repeats. Existing as an unconventional Myosin, Myosin XVI is involved in intracellular movements related to Actin filaments and is thought to interact specifically with PP1 alpha and PP1 gamma, possibly playing a role in PP1-associated brain development. Multiple isoforms of Myosin XVI exist due to alternative splicing events.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human MYO16
Antigen Species
Human
Gene Name
MYO16
Gene ID
Protein Name
Unconventional myosin-XVI
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 206 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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