Anti-MYL9 Antibody (5P798) is a Rabbit antibody targeting MYL9. Anti-MYL9 Antibody (5P798) can be used in FCM, IF, IHC-Fr, IHC-P, IP, WB.

IgG

5P798

Human,Mouse (predicted:Rat)

1. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MYL9 Monoclonal Antibody, Unconjugated (TMAB-09159) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MYL9 Monoclonal Antibody, Unconjugated (TMAB-09159) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MYL9 Monoclonal Antibody, Unconjugated (TMAB-09159) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. 25 ug total protein per lane of various lysates (see on figure) probed with MYL9 monoclonal antibody, unconjugated (TMAB-09159) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
5. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-MYL9 antibody (TMAB-09159, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

FCM=1:50-100; IF=1:50-200; IHC-Fr=1:50-200; IHC-P=1:50-200; IP=1:20-50; WB=1:500-2000

Monoclonal

A synthesized peptide: human Myosin light chain 9

Human

MYL9

Myosin regulatory light polypeptide 9

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion (PubMed:11942626, PubMed:2526655).<br>In myoblasts, may regulate PIEZO1-dependent cortical actomyosin assembly involved in myotube formation (By similarity).