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Anti-MYH6 Antibody (2D813) is a Rabbit antibody targeting MYH6. Anti-MYH6 Antibody (2D813) can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $148 | 7-10 days | 7-10 days | |
| 50 μL | $261 | 7-10 days | 7-10 days | |
| 100 μL | $472 | 7-10 days | 7-10 days |
| Description | Anti-MYH6 Antibody (2D813) is a Rabbit antibody targeting MYH6. Anti-MYH6 Antibody (2D813) can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Clone | 2D813 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. 25 μg total protein per lane of various lysates (see on figure) probed with MYH6 monoclonal antibody, unconjugated (TMAB-09150) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 2. Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MYH6 Monoclonal Antibody, Unconjugated (TMAB-09150) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MYH6 Monoclonal Antibody, Unconjugated (TMAB-09150) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MYH6 Monoclonal Antibody, Unconjugated (TMAB-09150) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MYH6 Monoclonal Antibody, Unconjugated (TMAB-09150) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm, myofibril. Note=Thick filaments of the myofibrils. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.02% Proclin300. |
| Concentration | 1mg/ml |
| Research Background | Cardiac muscle myosin is a hexamer consisting of two heavy chain subunits, two light chain subunits, and two regulatory subunits. This gene encodes the alpha heavy chain subunit of cardiac myosin. The gene is located approximately 4kb downstream of the gene encoding the beta heavy chain subunit of cardiac myosin. Mutations in this gene cause familial hypertrophic cardiomyopathy and atrial septal defect 3. [provided by RefSeq, Feb 2017] |
| Immunogen | A synthesized peptide: human Myosin heavy chain 6 |
| Antigen Species | Human |
| Gene Name | MYH6 |
| Gene ID | |
| Protein Name | Myosin-6 |
| Uniprot ID | |
| Function | Muscle contraction. |
| Molecular Weight | Theoretical: 224 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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