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Anti-MYF5 Polyclonal Antibody 2

Catalog No. TMAB-09147 Copy Product Info
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Anti-MYF5 Polyclonal Antibody 2 is a Rabbit antibody targeting MYF5. Anti-MYF5 Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,IF.

Anti-MYF5 Polyclonal Antibody 2

Copy Product Info
🥰Excellent
Catalog No. TMAB-09147

Anti-MYF5 Polyclonal Antibody 2 is a Rabbit antibody targeting MYF5. Anti-MYF5 Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,IF.

Anti-MYF5 Polyclonal Antibody 2
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2217-10 days7-10 days
100 μL$3727-10 days7-10 days
200 μL$5277-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-MYF5 Polyclonal Antibody 2 is a Rabbit antibody targeting MYF5. Anti-MYF5 Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Mouse (predicted:Human,Rat)
Verified Activity
Paraformaldehyde-fixed, paraffin embedded (mouse embryos tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MYF5) Polyclonal Antibody, Unconjugated (TMAB-09147) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundDifferentiation of myogenic cells is regulated by multiple positively and negatively acting factors. One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development include Myo D, myogenin, Myf-5 and Myf-6 (also designated MRF-4 or herculin). Of interest, most muscle cells express either Myo D or Myf-5 in the committed state, but when induced to differentiate, all turn on expression of myogenin. Myo D transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, the E family, which consists of at least three distinct genes: E2A, IF2 and HEB. Myo D-E heterodimers bind avidly to consensus (CANNTG) E box target sites that are functionally important elements in the upstream regulatory sequences of many muscle-specific terminal differentiation genes.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human MYF5
Antigen Species
Human
Gene Name
MYF5
Gene ID
Protein Name
Myogenic factor 5
Uniprot ID
Function
Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
Chemical Properties
Molecular WeightTheoretical: 28 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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