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Anti-MYF5 Polyclonal Antibody (Synonyms: Myogenic factor 5, Myf-5, Class C basic helix-loop-helix protein 2, ...)
Catalog No. TMAB-01193 Copy Product Info
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Anti-MYF5 Polyclonal Antibody is a Rabbit antibody targeting MYF5. Anti-MYF5 Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P, WB.
Anti-MYF5 Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-01193
Synonyms Myogenic factor 5, Myf-5, Class C basic helix-loop-helix protein 2, Class C basic helix loop helix protein 2, bHLHc2
Anti-MYF5 Polyclonal Antibody is a Rabbit antibody targeting MYF5. Anti-MYF5 Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P, WB.
Anti-MYF5 Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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| Description | Anti-MYF5 Polyclonal Antibody is a Rabbit antibody targeting MYF5. Anti-MYF5 Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P, WB. |
| Synonyms | Myogenic factor 5, Myf-5, Class C basic helix-loop-helix protein 2, Class C basic helix loop helix protein 2, bHLHc2 |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Chicken,Pig,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (mouse embryos tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MYF5) Polyclonal Antibody, Unconjugated (TMAB-01193) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Lane 1: Muscle (Mouse) Lysate at 40 μg Lane 2: Tongue (Mouse) Lysate at 40 μg Lane 3: Muscle (Rat) Lysate at 40 μg Lane 4: Tongue (Rat) Lysate at 40 μg Primary: Anti-MYF5 (TMAB-01193) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kDa Observed band size: 28 kDa 3. Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MYF5) Polyclonal Antibody, Unconjugated (TMAB-01193) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (Rat tongue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MYF5) Polyclonal Antibody, Unconjugated (TMAB-01193) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.     |
| Application | |
| Recommended Dose | IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500; WB=1:500-2000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | Differentiation of myogenic cells is regulated by multiple positively and negatively acting factors. One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development include Myo D, myogenin, Myf-5 and Myf-6 (also designated MRF-4 or herculin). Of interest, most muscle cells express either Myo D or Myf-5 in the committed state, but when induced to differentiate, all turn on expression of myogenin. Myo D transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, the E family, which consists of at least three distinct genes: E2A, IF2 and HEB. Myo D-E heterodimers bind avidly to consensus (CANNTG) E box target sites that are functionally important elements in the upstream regulatory sequences of many muscle-specific terminal differentiation genes. |
| Immunogen | KLH conjugated synthetic peptide: human MYF5 |
| Antigen Species | Human |
| Gene Name | MYF5 |
| Gene ID | |
| Protein Name | Myogenic factor 5 |
| Uniprot ID | |
| Biology Area | Myogenesis,Transcription Factors,Helix-Turn-Helix,Muscle |
| Function | Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein. |
| Molecular Weight | Theoretical: 28 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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