Shopping Cart
Remove All
Your shopping cart is currently empty
Anti-MYF5 Polyclonal Antibody
😃Good
Catalog No. TMAB-01193
Alias Myogenic factor 5, Myf-5, Class C basic helix-loop-helix protein 2, Class C basic helix loop helix protein 2, bHLHc2
Anti-MYF5 Polyclonal Antibody is a Rabbit antibody targeting MYF5. Anti-MYF5 Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P, WB.
Anti-MYF5 Polyclonal Antibody
Anti-MYF5 Polyclonal Antibody
😃Good
Catalog No. TMAB-01193Alias Myogenic factor 5, Myf-5, Class C basic helix-loop-helix protein 2, Class C basic helix loop helix protein 2, bHLHc2
Anti-MYF5 Polyclonal Antibody is a Rabbit antibody targeting MYF5. Anti-MYF5 Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P, WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
Add to Cart
Add to Quotation
In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
Resource Download
Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-MYF5 Polyclonal Antibody is a Rabbit antibody targeting MYF5. Anti-MYF5 Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P, WB. |
| Synonyms | Myogenic factor 5, Myf-5, Class C basic helix-loop-helix protein 2, Class C basic helix loop helix protein 2, bHLHc2 |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Chicken,Pig,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (mouse embryos tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MYF5) Polyclonal Antibody, Unconjugated (TMAB-01193) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Lane 1: Muscle (Mouse) Lysate at 40 μg Lane 2: Tongue (Mouse) Lysate at 40 μg Lane 3: Muscle (Rat) Lysate at 40 μg Lane 4: Tongue (Rat) Lysate at 40 μg Primary: Anti-MYF5 (TMAB-01193) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kDa Observed band size: 28 kDa 3. Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MYF5) Polyclonal Antibody, Unconjugated (TMAB-01193) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (Rat tongue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MYF5) Polyclonal Antibody, Unconjugated (TMAB-01193) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.     |
| Application | |
| Recommended Dose | IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500; WB=1:500-2000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | Differentiation of myogenic cells is regulated by multiple positively and negatively acting factors. One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development include Myo D, myogenin, Myf-5 and Myf-6 (also designated MRF-4 or herculin). Of interest, most muscle cells express either Myo D or Myf-5 in the committed state, but when induced to differentiate, all turn on expression of myogenin. Myo D transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, the E family, which consists of at least three distinct genes: E2A, IF2 and HEB. Myo D-E heterodimers bind avidly to consensus (CANNTG) E box target sites that are functionally important elements in the upstream regulatory sequences of many muscle-specific terminal differentiation genes. |
| Immunogen | KLH conjugated synthetic peptide: human MYF5 |
| Antigen Species | Human |
| Gene Name | MYF5 |
| Gene ID | |
| Protein Name | Myogenic factor 5 |
| Uniprot ID | |
| Biology Area | Myogenesis,Transcription Factors,Helix-Turn-Helix,Muscle |
| Function | Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein. |
| Molecular Weight | Theoretical: 28 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Calculator
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Preview
Related Tags: buy Anti-MYF5 Polyclonal Antibody | purchase Anti-MYF5 Polyclonal Antibody | Anti-MYF5 Polyclonal Antibody cost | order Anti-MYF5 Polyclonal Antibody | Anti-MYF5 Polyclonal Antibody molecular weight
Generate Quote
Catalog No.: Cas No.:
Add
| Size | Quantity | Unit Price | Amount | Operation |
|---|
Generate Quote
- TOP
Feedback
Hello! How can I help you today? 
Copyright © 2015-2026 TargetMol Chemicals Inc. All Rights Reserved.