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Anti-Myc Tag Antibody (6S669)
😃Good
Catalog No. TMAY-00032
Anti-Myc Tag Antibody (6S669) is a Mouse antibody targeting Myc Tag. Anti-Myc Tag Antibody (6S669) can be used in IP,WB.
Anti-Myc Tag Antibody (6S669)
Anti-Myc Tag Antibody (6S669)
😃Good
Catalog No. TMAY-00032
Anti-Myc Tag Antibody (6S669) is a Mouse antibody targeting Myc Tag. Anti-Myc Tag Antibody (6S669) can be used in IP,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 20 μL | $92 | 7-10 days | 7-10 days | |
| 100 μL | $186 | 7-10 days | 7-10 days | |
| 500 μL | $469 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-Myc Tag Antibody (6S669) is a Mouse antibody targeting Myc Tag. Anti-Myc Tag Antibody (6S669) can be used in IP,WB. |
| Ig Type | Mouse IgG1 |
| Clone | 6S669 |
| Reactivity | other |
| Specificity | Recognize N-terminal, C-terminal in fusion proteins. |
| Verified Activity | 1. Anti-Myc Tag mouse monoclonal antibody at 1:5000 dilution. -Lane A: GST-myc(Recombinant protein) (5ng) -Lane B: GST-myc(Recombinant protein) (1ng) -Lane C: myc-GST(Recombinant protein) (100ng) -Lane D: myc-GST(Recombinant protein) (20ng) -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. 2. Anti-Myc Tag mouse monoclonal antibody at 1:5000 dilution. -Lane A: HA-mFABP4-myc transfected 293 cell lysate (10ug) -Lane B: myc-ARG1-HA transfected 293 cell lysate (5ug) -Lane C: HA-ARG1-myc transfected 293 cell lysate (10ug) -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. 3. MYC was immunoprecipitated using: -Lane A:0.5 mg HA-ARG1-myc Whole Cell Lysate. -Lane B:0.5 mg myc-ARG1-HA Whole Cell Lysate. -4 µL anti-MYC mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G. -Primary antibody: -Anti-MYC mouse monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size: 40 kDa. -Observed band size:40 kDa |
| Application | |
| Recommended Dose | WB: 1:5000-1:10000; IP: 1-5 µL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, a synthetic Myc tag peptide conjugated to KLH. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | The Myc tag is a popular protein tag derived from c-myc gene product with a molecular weight of 1.2 KDa. The amino acid sequence is EQKLISEEDL, corresponding to the C-terminal amino acids (410-419) of human c-myc protein. Anti-Myc Tag antibodies can facilitate the detection, separation and purification of Myc tag target proteins without protein-specific antibodies or probes in several immunochemical assays such as Western Blotting, ELISAs and immunostaining techniques. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Myc tag peptide conjugated to KLH |
| Antigen Species | other |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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