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Anti-Myc Tag Antibody (2E237)

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Catalog No. TMAY-00031

Anti-Myc Tag Antibody (2E237) is a Mouse antibody targeting Myc Tag. Anti-Myc Tag Antibody (2E237) can be used in IP.

Anti-Myc Tag Antibody (2E237)

Anti-Myc Tag Antibody (2E237)

😃Good
Catalog No. TMAY-00031
Anti-Myc Tag Antibody (2E237) is a Mouse antibody targeting Myc Tag. Anti-Myc Tag Antibody (2E237) can be used in IP.
Pack SizePriceAvailabilityQuantity
20 μL$94 7-10 days
100 μL$186 7-10 days
500 μL$468 7-10 days
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Product Introduction

Bioactivity
Description
Anti-Myc Tag Antibody (2E237) is a Mouse antibody targeting Myc Tag. Anti-Myc Tag Antibody (2E237) can be used in IP.
Ig Type
Monoclonal Mouse IgG1
Clone
2E237
Reactivity
other
Verified Activity
MYC tag was immunoprecipitated using:

-Lane A:0.5 mg myc-ARG1-HA transfected 293 Whole Cell Lysate.

-Lane B:0.5 mg HA-ARG1-myc transfected 293 Whole Cell Lysate.

-2 µL anti-MYC tag mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-MYC tag mouse monoclonal antibody, at 1:100 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 39 kDa.

-Observed band size: 39 kDa
Application
IP
Recommended Dose
IP: 1-4 μg/mg of lysate
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, a synthetic Myc tag peptide conjugated to KLH. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundThe Myc tag is a popular protein tag derived from c-myc gene product with a molecular weight of 1.2 KDa. The amino acid sequence is EQKLISEEDL, corresponding to the C-terminal amino acids (410-419) of human c-myc protein. Anti-Myc Tag antibodies can facilitate the detection, separation and purification of Myc tag target proteins without protein-specific antibodies or probes in several immunochemical assays such as Western Blotting, ELISAs and immunostaining techniques.
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
HRP
Antibody Types Available
2
Antigen Details
Immunogen
A synthetic Myc tag peptide conjugated to KLH
Antigen Species
other
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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