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Anti-Myc Tag Antibody (2E237) is a Mouse antibody targeting Myc Tag. Anti-Myc Tag Antibody (2E237) can be used in IP.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
20 μL | $94 | 7-10 days | |
100 μL | $186 | 7-10 days | |
500 μL | $468 | 7-10 days |
Description | Anti-Myc Tag Antibody (2E237) is a Mouse antibody targeting Myc Tag. Anti-Myc Tag Antibody (2E237) can be used in IP. |
Ig Type | Monoclonal Mouse IgG1 |
Clone | 2E237 |
Reactivity | other |
Verified Activity | MYC tag was immunoprecipitated using: -Lane A:0.5 mg myc-ARG1-HA transfected 293 Whole Cell Lysate. -Lane B:0.5 mg HA-ARG1-myc transfected 293 Whole Cell Lysate. -2 µL anti-MYC tag mouse monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-MYC tag mouse monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 39 kDa. -Observed band size: 39 kDa |
Application | IP |
Recommended Dose | IP: 1-4 μg/mg of lysate |
Antibody Type | Monoclonal |
Host Species | Mouse |
Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, a synthetic Myc tag peptide conjugated to KLH. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Research Background | The Myc tag is a popular protein tag derived from c-myc gene product with a molecular weight of 1.2 KDa. The amino acid sequence is EQKLISEEDL, corresponding to the C-terminal amino acids (410-419) of human c-myc protein. Anti-Myc Tag antibodies can facilitate the detection, separation and purification of Myc tag target proteins without protein-specific antibodies or probes in several immunochemical assays such as Western Blotting, ELISAs and immunostaining techniques. |
Conjucates | Unconjugated |
Others Formats | HRP |
Antibody Types Available | 2 |
Immunogen | A synthetic Myc tag peptide conjugated to KLH |
Antigen Species | other |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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