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Anti-MXRA8 Polyclonal Antibody

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Catalog No. TMAB-01188

Anti-MXRA8 Polyclonal Antibody is a Rabbit antibody targeting MXRA8. Anti-MXRA8 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-MXRA8 Polyclonal Antibody

Anti-MXRA8 Polyclonal Antibody

😃Good
Catalog No. TMAB-01188
Anti-MXRA8 Polyclonal Antibody is a Rabbit antibody targeting MXRA8. Anti-MXRA8 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$222 7-10 days
100 μL$373 7-10 days
200 μL$528 7-10 days
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Product Introduction

Bioactivity
Description
Anti-MXRA8 Polyclonal Antibody is a Rabbit antibody targeting MXRA8. Anti-MXRA8 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Dog,Pig,Cow,Sheep)
Verified Activity
1. Sample:
Ovary (Mouse) Lysate at 40 μg
MCF-7 (Human) Cell Lysate at 30 μg
MD-MB-231 (Human) Cell Lysate at 30 μg
Primary: Anti-MXRA8 (TMAB-01188) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
2. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MXRA8) Polyclonal Antibody, Unconjugated (TMAB-01188) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MXRA8) Polyclonal Antibody, Unconjugated (TMAB-01188) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MXRA8) Polyclonal Antibody, Unconjugated (TMAB-01188) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
5. Sample:
Lane 1: Human U-2 OS cell lysates
Lane 2: Human HepG2 cell lysates
Primary: Anti-MXRA8 (TMAB-01188) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 56 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundMXRA8 is a 442 amino acid single-pass type I membrane protein that is implicated in the maintenance and maturation of the blood-brain barrier. Containing two Ig-like V-type (immunoglobulin-like) domains, MXRA8 exists as two alternatively spliced isoforms that are encoded by a gene that maps to human chromosome 1p36.33. Human chromosome 1 spans 260 million base pairs, contains over 3,000 genes and comprises nearly 8% of the human genome. Chromosome 1 houses a large number of disease-associated genes, including those that are involved in familial adenomatous polyposis, Stickler syndrome, Parkinson’s disease, Gaucher disease, schizophrenia and Usher syndrome. Aberrations in chromosome 1 are found in a variety of cancers, including head and neck cancer, malignant melanoma and multiple myeloma
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human MXRA8
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Other Antibodies
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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