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Anti-Mucin-2/MUC2 Antibody (1B747)

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Catalog No. TMAB-01186

Anti-Mucin-2/MUC2 Antibody (1B747) is a Rabbit antibody targeting Mucin-2/MUC2. Anti-Mucin-2/MUC2 Antibody (1B747) can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Anti-Mucin-2/MUC2 Antibody (1B747)

Anti-Mucin-2/MUC2 Antibody (1B747)

😃Good
Catalog No. TMAB-01186
Anti-Mucin-2/MUC2 Antibody (1B747) is a Rabbit antibody targeting Mucin-2/MUC2. Anti-Mucin-2/MUC2 Antibody (1B747) can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
25 μL$153 7-10 days
50 μL$272 7-10 days
100 μL$489 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Rabbit Monoclonal

Application: WB,IHC-P,IHC-Fr,IF,FCM

Reactivity: Human,Rat
Ig Type
IgG
Clone
1B747
Reactivity
Human,Rat
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (MUC2) Monoclonal Antibody, Unconjugated (TMAB-01186) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (MUC2) Monoclonal Antibody, Unconjugated (TMAB-01186) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Human duodenum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (MUC2) Monoclonal Antibody, Unconjugated (TMAB-01186) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC2 Monoclonal Antibody, Unconjugated (TMAB-01186) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
5. 25 μg total protein per Lane of various lysates probed with MUC2 monoclonal antibody, unconjugated (TMAB-01186) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
Application
WB,IHC-P,IHC-Fr,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted. Note=In the intestine, secreted into the inner and outer mucus layers.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. The protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human MUC2
Antigen Species
Human
Gene ID
4583
Uniprot ID
Biology Area
MUC,Tumor-associated antigens,MUC,Tumor Associated
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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