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Anti-MUC13 Polyclonal Antibody

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Catalog No. TMAB-09093

Anti-MUC13 Polyclonal Antibody is a Rabbit antibody targeting MUC13. Anti-MUC13 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-MUC13 Polyclonal Antibody

Anti-MUC13 Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-09093
Anti-MUC13 Polyclonal Antibody is a Rabbit antibody targeting MUC13. Anti-MUC13 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2217-10 days7-10 days
100 μL$3747-10 days7-10 days
200 μL$5287-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-MUC13 Polyclonal Antibody is a Rabbit antibody targeting MUC13. Anti-MUC13 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Rat (predicted:Mouse,Pig,Horse)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MUC13) Polyclonal Antibody, Unconjugated (TMAB-09093) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Sample:
Lane 1: Human HeLa cell lysates
Lane 2: Human HepG2 cell lysates
Lane 3: Human A549 cell lysates
Primary: Anti-MUC13 (TMAB-09093) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 120 kDa
3. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (MUC13) Polyclonal Antibody, Unconjugated (TMAB-09093) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Single-pass type I membrane protein (Potential). Secreted. Note=Also exists as a soluble form.
Tissue SpecificityHighly expressed in epithelial tissues, particularly those of the gastrointestinal and respiratory tracts, such as large intestine and trachea, followed by kidney, small intestine, appendix and stomach.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundMUC13 is a mucin. These are secreted or transmembrane glycoproteins that are expressed mainly in the digestive tract and on epithelial surfaces. MUC13 is upregulated in gastric cancer. Mucins have also been implicated in inflammatory bowel diseases.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human MUC13
Antigen Species
Human
Gene Name
MUC13
Gene ID
Protein Name
Mucin-13
Uniprot ID
Function
Epithelial and hemopoietic transmembrane mucin that may play a role in cell signaling.
Chemical Properties
Molecular WeightTheoretical: 54 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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