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Anti-MUC13 Polyclonal Antibody is a Rabbit antibody targeting MUC13. Anti-MUC13 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
| Description | Anti-MUC13 Polyclonal Antibody is a Rabbit antibody targeting MUC13. Anti-MUC13 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse,Pig,Horse) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MUC13) Polyclonal Antibody, Unconjugated (TMAB-09093) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Sample: Lane 1: Human HeLa cell lysates Lane 2: Human HepG2 cell lysates Lane 3: Human A549 cell lysates Primary: Anti-MUC13 (TMAB-09093) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 120 kDa 3. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (MUC13) Polyclonal Antibody, Unconjugated (TMAB-09093) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Single-pass type I membrane protein (Potential). Secreted. Note=Also exists as a soluble form. |
| Tissue Specificity | Highly expressed in epithelial tissues, particularly those of the gastrointestinal and respiratory tracts, such as large intestine and trachea, followed by kidney, small intestine, appendix and stomach. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | MUC13 is a mucin. These are secreted or transmembrane glycoproteins that are expressed mainly in the digestive tract and on epithelial surfaces. MUC13 is upregulated in gastric cancer. Mucins have also been implicated in inflammatory bowel diseases. |
| Immunogen | KLH conjugated synthetic peptide: human MUC13 |
| Antigen Species | Human |
| Gene Name | MUC13 |
| Gene ID | |
| Protein Name | Mucin-13 |
| Uniprot ID | |
| Function | Epithelial and hemopoietic transmembrane mucin that may play a role in cell signaling. |
| Molecular Weight | Theoretical: 54 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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