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Anti-MTHFD1 Antibody (1C524)

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Catalog No. TMAB-09064

Anti-MTHFD1 Antibody (1C524) is a Mouse antibody targeting MTHFD1. Anti-MTHFD1 Antibody (1C524) can be used in WB,ICC/IF.

Anti-MTHFD1 Antibody (1C524)

Anti-MTHFD1 Antibody (1C524)

Copy Product Info
😃Good
Catalog No. TMAB-09064
Anti-MTHFD1 Antibody (1C524) is a Mouse antibody targeting MTHFD1. Anti-MTHFD1 Antibody (1C524) can be used in WB,ICC/IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1517-10 days7-10 days
50 μL$2627-10 days7-10 days
100 μL$4717-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-MTHFD1 Antibody (1C524) is a Mouse antibody targeting MTHFD1. Anti-MTHFD1 Antibody (1C524) can be used in WB,ICC/IF.
Ig Type
IgG1, Kappa
Clone
1C524
Reactivity
Human (predicted:Mouse)
Verified Activity
1. Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: room temperature 2 h
Secondary Ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 1: HeLa, 2: 293, 3: A20, 4: Raw264.7
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 102 kDa
Observed MW: 102 kDa
2. Cell line: HEK-293
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-09064
Application
Recommended Dose
WB: 1:500-1000; ICC/IF: 1:100-500
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationCytoplasm.
Tissue SpecificityUbiquitous.
ConstructionRecombinant Antibody
PurificationProtein G purified
AppearanceLiquid
FormulationPBS, Glycerol, BSA.
Concentration1mg/ml
Research BackgroundThis gene encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain. [provided by RefSeq, Jul 2008]
Antigen Details
Gene Name
MTHFD1
Gene ID
Protein Name
C-1-tetrahydrofolate synthase, cytoplasmic
Chemical Properties
Molecular WeightTheoretical: 110 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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