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Anti-MT2A Polyclonal Antibody is a Rabbit antibody targeting MT2A. Anti-MT2A Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $374 | 7-10 days | |
200 μL | $529 | 7-10 days |
Description | Anti-MT2A Polyclonal Antibody is a Rabbit antibody targeting MT2A. Anti-MT2A Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM. |
Ig Type | IgG |
Reactivity | Human,Mouse (predicted:Rat) |
Verified Activity | 1. Tissue/cell: mouse colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Metallothionein Polyclonal Antibody, Unconjugated (TMAB-01174) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Metallothionein) Polyclonal Antibody, Unconjugated (TMAB-01174) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Blank control: U937. Primary Antibody (green line): Rabbit Anti-Metallothionein antibody (TMAB-01174) Dilution: 1 μg/Test; Secondary Antibody: Goat anti-rabbit IgG-FITC Dilution: 0.5 μg/Test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. |
Application | IHC-P,IHC-Fr,IF,FCM |
Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | Metallothioneins (MTs) are a family of low molecular weight, heavy metal-binding proteins characterized by a high cysteine content and lack of aromatic amino acids. MTs bind 7 to 12 heavy metal atoms per molecule of protein. They are ubiquitous in the animal and plant kingdoms and are also found in prokaryotes. In mammals, the cysteine residues are absolutely conserved and serve to coordinate heavy metal atoms such as zinc, cadmium, and copper via mercaptide linkages. |
Immunogen | KLH conjugated synthetic peptide: mouse MT2A |
Antigen Species | Mouse |
Gene ID | |
Uniprot ID |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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