Anti-MT-ND6 Polyclonal Antibody

Anti-MT-ND6 Polyclonal Antibody is a Rabbit antibody targeting MT-ND6. Anti-MT-ND6 Polyclonal Antibody can be used in FCM,IF,IHC-Fr,IHC-P.

IgG

Human,Rat (predicted:Mouse,Dog,Cow,Horse,Sheep)

1. Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-MT-ND6 antibody (TMAB-01176)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MT-ND6) Polyclonal Antibody, Unconjugated (TMAB-01176) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: human MTND6

Human

MT-ND6

NADH-ubiquinone oxidoreductase chain 6

Integration of energy metabolism,Integration of energy,Mitochondrial markers,Complex I,Visual system,Mitochondrial

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).