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Anti-MT-ND6 Polyclonal Antibody

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Catalog No. TMAB-01176

Anti-MT-ND6 Polyclonal Antibody is a Rabbit antibody targeting MT-ND6. Anti-MT-ND6 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

Anti-MT-ND6 Polyclonal Antibody

Anti-MT-ND6 Polyclonal Antibody

😃Good
Catalog No. TMAB-01176
Anti-MT-ND6 Polyclonal Antibody is a Rabbit antibody targeting MT-ND6. Anti-MT-ND6 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$221 7-10 days
100 μL$374 7-10 days
200 μL$529 7-10 days
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Product Introduction

Bioactivity
Description
Anti-MT-ND6 Polyclonal Antibody is a Rabbit antibody targeting MT-ND6. Anti-MT-ND6 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Ig Type
IgG
Reactivity
Human,Rat (predicted:Mouse,Dog,Cow,Horse,Sheep)
Verified Activity
1. Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-MT-ND6 antibody (TMAB-01176)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MT-ND6) Polyclonal Antibody, Unconjugated (TMAB-01176) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Application
IHC-P,IHC-Fr,IF,FCM
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMitochondrion membrane; Multi-pass membrane protein (Potential).
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundNADH Dehydrogenase subunit 6 (MTND6) is 1 of the 7 mitochondrial DNA (mtDNA) encoded subunits (MTND1, MTND2, MTND3, MTND4L, MTND4, MTND5, MTND6) included among the approximately 41 polypeptides of respiratory Complex I. Complex I accepts electrons from NADH, transfers them to ubiquinone (Coenzyme Q10), and uses the energy released to pump protons across the mitochondria inner membrane. MTND6 has been proposed to be a component of the iron-protein fragment.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human MTND6
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Integration of energy metabolism,Integration of energy,Mitochondrial markers,Complex I,Visual system,Mitochondrial
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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