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Anti-MSH6 Antibody (6V732) is a Rabbit antibody targeting MSH6. Anti-MSH6 Antibody (6V732) can be used in WB,ICC/IF,IHC.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $297 | 7-10 days | |
100 μL | $498 | 7-10 days |
Description | Anti-MSH6 Antibody (6V732) is a Rabbit antibody targeting MSH6. Anti-MSH6 Antibody (6V732) can be used in WB,ICC/IF,IHC. |
Synonyms | Sperm associated protein, p160, MutS-alpha 160 kDa subunit, mutS homolog 6, MutS alpha 160 kDa subunit, mutS (E. coli) homolog 6, MSH 6, HSAP, HNPCC5, HNPCC 5, hMSH6, GTMBP, GTBP, G/T mismatch binding protein, DNA mismatch repair protein Msh6 |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Western blot analysis of MSH6 on different lysates using anti-MSH6 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2, Lane 2: SW480, Lane 3: A549. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MSH6 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-MSH6 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-MSH6 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-MSH6 antibody. Counter stained with hematoxylin. 6. ICC staining MSH6 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining MSH6 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Application | |
Recommended Dose | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | Multiple pathways promote short-sequence recombination (SSR) in Saccharomyces cerevisiae. When gene conversion is initiated by a double-strand break (DSB), any nonhomologous DNA that may be present at the ends must be removed before new DNA synthesis can be initiated. Removal of a 3' nonhomologous tail in S. cerevisiae depends on the nucleotide excision repair endonuclease Rad1/Rad10 and also on the mismatch repair proteins Msh2 and Msh3. Msh2 and Msh3, which function in mitotic recombination, recognize not only heteroduplex loops and mismatched basepairs, but also branched DNA structures with a free 3' tail. Msh2 and Msh6 form a protein complex required to repair mismatches generated during DNA replication. Yeast Msh2-Msh6 interact asymmetrically with the DNA through base-specific stacking and hydrogen bonding interactions and backbone contacts. The importance of these contacts decreases with increasing distance from the mismatch, implying that interactions at or near the mismatch are important for binding in a kinked DNA conformation. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein |
Uniprot ID |
Molecular Weight | Theoretical: 153 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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