Antibody Type: Rabbit Polyclonal

Application: WB,ICC/IF,FCM

Reactivity: Human

IgG

Human

1. 25 μg total protein per Lane of various lysates probed with MRC1 polyclonal antibody, unconjugated (TMAB-01167) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
2. 4% Paraformaldehyde-fixed Molt-4 (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (MRC1) polyclonal Antibody, unconjugated (TMAB-01167) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green ) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control.
3. The Molt-4 (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at room temperature). Primary Antibody (green): Rabbit Anti-MRC1 antibody (TMAB-01167): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS.

WB,ICC/IF,FCM

WB: 1:500-2000; ICC/IF: 1:100; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: human MRC1/CD206

Human

4360

Receptors,Macrophage / Inflamm.