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Anti-MRC1 Antibody (6S133) is a Rabbit antibody targeting MRC1. Anti-MRC1 Antibody (6S133) can be used in IHC-P,IHC-Fr,ICC/IF,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
25 μL | $154 | 7-10 days | |
50 μL | $272 | 7-10 days | |
100 μL | $489 | 7-10 days |
Description | Antibody Type: Recombinant Rabbit Monoclonal Application: IHC-P,IHC-Fr,ICC/IF,IF Reactivity: Human |
Ig Type | IgG |
Clone | 6S133 |
Reactivity | Human |
Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD206) Monoclonal Antibody, Unconjugated (TMAB-01165) at 1:300 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD206) Monoclonal Antibody, Unconjugated (TMAB-01165) at 1:300 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD206) Monoclonal Antibody, Unconjugated (TMAB-01165) at 1:300 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD206) Monoclonal Antibody, Unconjugated (TMAB-01165) at 1:300 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD206 Monoclonal Antibody, Unconjugated (TMAB-01165) at 1:1000 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD206 Monoclonal Antibody, Unconjugated (TMAB-01165) at 1:1000 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD206 Monoclonal Antibody, Unconjugated (TMAB-01165) at 1:1000 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 8. 4% Paraformaldehyde-fixed Molt-4 (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (MRC1) monoclonal Antibody, unconjugated (TMAB-01165) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control. |
Application | IHC-P,IHC-Fr,ICC/IF,IF |
Recommended Dose | IHC-P: 1:200-1000; IHC-Fr: 1:200-1000; ICC/IF: 1:50-200; IF: 1:200-1000 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Subcellular Localization | Endosome membrane; Single-pass type I membrane protein. Cell membrane; Single-pass type I membrane protein. |
Construction | Recombinant Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | PBS, Glycerol, BSA. |
Research Background | The recognition of complex carbohydrate structures on glycoproteins is an important part of several biological processes, including cell-cell recognition, serum glycoprotein turnover, and neutralization of pathogens. The protein encoded by this gene is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. The protein has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. This gene is in close proximity to MRC1L1. The gene loci including this gene, MRC1L1, as well as LOC340843 and LOC340893, consist of two nearly identical, tandemly linked genomic regions, which are thought to be a part of a duplicated region. [provided by RefSeq]. |
Immunogen | KLH conjugated synthetic peptide: human CD206 |
Antigen Species | Human |
Gene ID | 4360 |
Uniprot ID | |
Biology Area | Receptors,Macrophage / Inflamm. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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