Anti-MPO Polyclonal Antibody 3 is a Rabbit antibody targeting MPO. Anti-MPO Polyclonal Antibody 3 can be used in WB,FCM.

IgG

Human

1. Blank control (black line): HL60.
Primary Antibody (green line): Rabbit Anti-MPO antibody (TMAB-08965)
Dilution: 1 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution:0.5 μg/Test.
Negative control (white blue line): PBS
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Sample:
Lane 1: Recombinant human MPO protein
Primary: Anti-MPO (TMAB-08965) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 84 kDa
Observed band size: 15 kDa

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

Recombinant Protein: human Myeloperoxidase

Human

MPO

Myeloperoxidase

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.