Anti-MPO Polyclonal Antibody 2 is a Rabbit antibody targeting MPO. Anti-MPO Polyclonal Antibody 2 can be used in ICC/IF,IF,IHC-Fr,IHC-P.

IgG

Human,Mouse (predicted:Rat,Dog,Horse,Rabbit,GuineaPig)

1. Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MPO) Polyclonal Antibody, Unconjugated (TMAB-01164) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Tissue/cell: mouse brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-MPO Polyclonal Antibody, Unconjugated (TMAB-01164) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml,blue) was used to stain the cell nucleus.
3. Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-MPO Polyclonal Antibody, Unconjugated (TMAB-01164) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Myeloperoxidase heavy chain

Human

MPO

Myeloperoxidase

Cellular metabolic process,Blood,Arterial,Pulmonary,Macrophage / Inflamm.,Cancer proteins ELISA kits,Cardiovascular ELISA kits,Antioxidants

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.