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Anti-MPO Antibody (4N671)

(Synonyms: myeloperoxidase) Copy Product Info
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Synonyms: myeloperoxidase

Catalog No. TMAC-02717 Copy Product Info
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Anti-MPO Antibody (4N671) is a Rabbit antibody targeting MPO. Anti-MPO Antibody (4N671) can be used in ICC/IF,IHC,WB.

Anti-MPO Antibody
(4N671)

Pack SizePriceUSA StockGlobal StockQuantity
50 µL$2977-10 days7-10 days
100 µL$4977-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-MPO Antibody (4N671) is a Rabbit antibody targeting MPO. Anti-MPO Antibody (4N671) can be used in ICC/IF,IHC,WB.
Synonymsmyeloperoxidase
Ig Type
IgG
Clone
4N671
Reactivity
Human
Verified Activity
1. Western blot analysis of Myeloperoxidase on HL-60 cell lysates using anti-Myeloperoxidase antibody at 1/1,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Myeloperoxidase antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Myeloperoxidase antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Myeloperoxidase antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Myeloperoxidase antibody. Counter stained with hematoxylin.
6. ICC staining Myeloperoxidase in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining Myeloperoxidase in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. ICC staining Myeloperoxidase in AGS cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Application
Recommended Dose
WB: 1:500-1000; IHC: 1:50-200; ICC/IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundThe heme protein myeloperoxidase (MPO) is a major component of azurophilic granules of neutrophils and polymorphonuclear leukocytes. Optimal oxygen-dependent microbiocidal activity depends on MPO as the critical enzyme for the generation of hypochlorous acid and other toxic oxygen products. The MPO precursor is synthesized during the promyelocytic stage of myeloid differentiation and is subsequently processed and transported intracellularly to the lysosomes. The precursor undergoes cotranslational N-linked glycosylation to produce a glycoprotein. Glucosidases in the endoplasmic reticulum (ER) or early cis Golgi convert the pro-MPO to a form which is sorted into a prelysosomal compartment, which undergoes final proteolytic maturation to native MPO, a pair of heavy-light protomers. In normal neutrophils, MPO is expressed as a dimer. Calreticulin, a calcium-binding protein residing in the ER, interacts specifically with fully glycosylated apopro-MPO. iMPO mRNA is abundant in human promyelocytic HL-60 and mouse myeloid leukemia NFS-60 cells. MPO is expressed at high levels in circulating neutrophils and monocytes but is not detectable in microglia, brain-specific macrophages or normal brain tissue.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 84 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: Anti-MPO Antibody (4N671) molecular weight