Anti-Mono-methyl-Histone H4 (Lys20) Antibody (6L374) is a Mouse antibody targeting Mono-methyl-Histone H4 (Lys20). Anti-Mono-methyl-Histone H4 (Lys20) Antibody (6L374) can be used in WB,ICC/IF.

IgG1, Kappa

6L374

Human (predicted:Mouse,Rat)

1. Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:100
Primary incubation condition: 1 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Comment: Color green is the positive signal for TMAB-08940
2. Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1: 2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: HeLa, N2a, BRL, Mouse spleen, Mouse kidney
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 11 kDa
Observed MW: 11 kDa

WB: 1:500-2000; ICC/IF: 1:50-100

Monoclonal

H4C1

Histone H4

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.