Anti-Mono-methyl-Histone H3 (Lys9) Polyclonal Antibody is a Rabbit antibody targeting Mono-methyl-Histone H3 (Lys9). Anti-Mono-methyl-Histone H3 (Lys9) Polyclonal Antibody can be used in WB,ICC/IF,IP.

IgG

Human,Mouse,Rat,Pharbitis,African Green Monkey

1. Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1: 200
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Comment: Color green is the positive signal for TMAB-08934
2. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1: 2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: HeLa, 3T3, COS
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 17 kDa
Observed MW: 17 kDa
3. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: Pharbitis, N2a, Mouse spleen
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 17 kDa
Observed MW: 17 kDa

WB: 1:500-2000; ICC/IF: 1:100-500; IP: 1:25-100

Polyclonal

H3C1

Histone H3.1

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. H3 is deposited into chromatin exclusively through a DNA replication-coupled pathway that can be associated with either DNA duplication or DNA repair synthesis during meiotic homologous recombination.