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Anti-MMP-12 Antibody (1B615)

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Catalog No. TMAC-02617
Alias MMP 12, hME

Anti-MMP-12 Antibody (1B615) is a Rabbit antibody targeting MMP-12. Anti-MMP-12 Antibody (1B615) can be used in WB,ICC/IF,IHC,IP,FCM.

Anti-MMP-12 Antibody (1B615)

Anti-MMP-12 Antibody (1B615)

😃Good
Catalog No. TMAC-02617Alias MMP 12, hME
Anti-MMP-12 Antibody (1B615) is a Rabbit antibody targeting MMP-12. Anti-MMP-12 Antibody (1B615) can be used in WB,ICC/IF,IHC,IP,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2987-10 days
100 μL$4987-10 days
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Product Introduction

Bioactivity
Description
Anti-MMP-12 Antibody (1B615) is a Rabbit antibody targeting MMP-12. Anti-MMP-12 Antibody (1B615) can be used in WB,ICC/IF,IHC,IP,FCM.
SynonymsMMP 12, hME
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. Western blot analysis of MMP12 on different cell lysates using anti-MMP12 antibody at 1/1,000 dilution. Positive control: Lane 1: A549, Lane 2: Hela, Lane 3: MCF-7, Lane 4: THP-1.
2. Immunohistochemical analysis of paraffin-embedded human breast carcinomna tissue using anti-MMP12 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-MMP12 antibody. Counter stained with hematoxylin.
4. Flow cytometric analysis of A549 cells with MMP12 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
Recommended Dose
WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundThe matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-12 (also designated macrophage metalloelastase) is produced in alveolar macrophages and degrades elastin. MMP-12 may contribute to elastin degradation occurring in granulomatous skin diseases and may also participate in macrophage migration through the epidermal and vascular basement membranes in inflammatory disorders.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 54/45/22 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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