Anti-MKLP1 Antibody (5H702) is a Rabbit antibody targeting MKLP1. Anti-MKLP1 Antibody (5H702) can be used in ICC/IF, WB.

IgG

5H702

Human,Mouse

1. Blank control: A549.
Primary Antibody (green line): Rabbit Anti-MKLP1 antibody (TMAB-08841)
Dilution: 2ul/Test;
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Sample:
Lane 1: Rat Lymph node tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Lane 3: Rat Testis tissue lysates
Primary: Anti-MKLP1 (TMAB-08841) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 110 kDa
Observed band size: 100 kDa

ICC/IF=1:100-500; WB=1:500-1000

Monoclonal

KLH conjugated synthetic peptide: human MKLP1

Human

KIF23

Kinesin-like protein KIF23

Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Essential for cytokinesis in Rho-mediated signaling. Required for the localization of ECT2 to the central spindle. Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro.