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Anti-MKI67 Polyclonal Antibody 2
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Catalog No. TMAB-01145
Anti-MKI67 Polyclonal Antibody 2 is a Rabbit antibody targeting MKI67. Anti-MKI67 Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,ICC/IF,IF.
Anti-MKI67 Polyclonal Antibody 2
Anti-MKI67 Polyclonal Antibody 2
😃Good
Catalog No. TMAB-01145
Anti-MKI67 Polyclonal Antibody 2 is a Rabbit antibody targeting MKI67. Anti-MKI67 Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,ICC/IF,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: IHC-P,IHC-Fr,ICC/IF,IF Reactivity: Human |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ki-67 Polyclonal Antibody, Unconjugated (TMAB-01145) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ki-67 Polyclonal Antibody, Unconjugated (TMAB-01145) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 3. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (Ki-67) polyclonal Antibody, unconjugated (TMAB-01145) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control. |
| Application | IHC-P,IHC-Fr,ICC/IF,IF |
| Recommended Dose | IHC-P: 1:200-800; IHC-Fr: 1:200-800; ICC/IF: 1:100; IF: 1:300-800 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Chromosome. Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation. |
| Immunogen | KLH conjugated synthetic peptide: human Ki-67 |
| Antigen Species | Human |
| Gene ID | 4288 |
| Uniprot ID | |
| Biology Area | Cell division,Tumor biomarkers,Markers,Soma marker,Neurogenesis,Replication |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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