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Anti-MITF Polyclonal Antibody is a Rabbit antibody targeting MITF. Anti-MITF Polyclonal Antibody can be used in WB,ICC/IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $221 | 7-10 days | |
100 μL | $373 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Anti-MITF Polyclonal Antibody is a Rabbit antibody targeting MITF. Anti-MITF Polyclonal Antibody can be used in WB,ICC/IF. |
Ig Type | IgG |
Reactivity | Human,Mouse (predicted:Rat,Chicken,Dog,Cow,Horse,Rabbit) |
Verified Activity | 1. Sample: Muscle (Mouse) Lysate at 40 μg Primary: Anti-MITF (TMAB-01139) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kDa Observed band size: 57 kDa 2. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (MITF) polyclonal Antibody, Unconjugated (TMAB-01139) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. |
Application | WB,ICC/IF |
Recommended Dose | WB: 1:500-2000; ICC/IF: 1:50-200 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Nucleus. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | The protein encoded by this gene is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. The encoded protein regulates melanocyte development and is responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. [provided by RefSeq, Aug 2017] |
Immunogen | KLH conjugated synthetic peptide: human MITF |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | HLH / Leucine Zipper,HLH,HLH / Leucine Zipper,Mast Cells |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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