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Anti-MITF Polyclonal Antibody

😃Good
Catalog No. TMAB-01139

Anti-MITF Polyclonal Antibody is a Rabbit antibody targeting MITF. Anti-MITF Polyclonal Antibody can be used in WB,ICC/IF.

Anti-MITF Polyclonal Antibody

Anti-MITF Polyclonal Antibody

😃Good
Catalog No. TMAB-01139
Anti-MITF Polyclonal Antibody is a Rabbit antibody targeting MITF. Anti-MITF Polyclonal Antibody can be used in WB,ICC/IF.
Pack SizePriceAvailabilityQuantity
50 μL$221 7-10 days
100 μL$373 7-10 days
200 μL$527 7-10 days
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Product Introduction

Bioactivity
Description
Anti-MITF Polyclonal Antibody is a Rabbit antibody targeting MITF. Anti-MITF Polyclonal Antibody can be used in WB,ICC/IF.
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Chicken,Dog,Cow,Horse,Rabbit)
Verified Activity
1. Sample:
Muscle (Mouse) Lysate at 40 μg
Primary: Anti-MITF (TMAB-01139) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kDa
Observed band size: 57 kDa
2. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (MITF) polyclonal Antibody, Unconjugated (TMAB-01139) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
Application
WB,ICC/IF
Recommended Dose
WB: 1:500-2000; ICC/IF: 1:50-200
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe protein encoded by this gene is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. The encoded protein regulates melanocyte development and is responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. [provided by RefSeq, Aug 2017]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human MITF
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
HLH / Leucine Zipper,HLH,HLH / Leucine Zipper,Mast Cells
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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