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Anti-MITF Antibody (9E208)
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Catalog No. TMAC-02589
Alias WS2A, WS2, MITF-A, Microphthalmia-associated transcription factor, MI, COMMAD, CMM8, Class E basic helix-loop-helix protein 32(bHLHe32), BHLHE32
Anti-MITF Antibody (9E208) is a Rabbit antibody targeting MITF. Anti-MITF Antibody (9E208) can be used in FCM,ICC,WB.
Anti-MITF Antibody (9E208)
Anti-MITF Antibody (9E208)
😃Good
Catalog No. TMAC-02589Alias WS2A, WS2, MITF-A, Microphthalmia-associated transcription factor, MI, COMMAD, CMM8, Class E basic helix-loop-helix protein 32(bHLHe32), BHLHE32
Anti-MITF Antibody (9E208) is a Rabbit antibody targeting MITF. Anti-MITF Antibody (9E208) can be used in FCM,ICC,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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Chemical Properties
| Description | Anti-MITF Antibody (9E208) is a Rabbit antibody targeting MITF. Anti-MITF Antibody (9E208) can be used in FCM,ICC,WB. |
| Synonyms | WS2A, WS2, MITF-A, Microphthalmia-associated transcription factor, MI, COMMAD, CMM8, Class E basic helix-loop-helix protein 32(bHLHe32), BHLHE32 |
| Ig Type | IgG |
| Clone | 9E208 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of MiTF on PC-12 cells lysates using anti-MiTF antibody at 1/1,000 dilution. 2. ICC staining MiTF in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining MiTF in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining MiTF in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of SW480 cells with MiTF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; ICC: 1:100-500; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | MITF (microphthalmia-associated transcription factor) is a melanocytic nuclear protein that contains basic helix-loop-helix (HLH) and leucine zipper (LZ) domains. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. MITF can directly associate with DNA as a homodimer and is required for the development and differentiation of melanocytes. Its expression is upregulated by cAMP and cAMP-dependent pathways. MITF activates several different gene promoters by binding to their E-boxes. Tyrosinase, TRP1 and TRP2 are pigment synthesis genes activated by MITF. When MITF is phosphorylated on Ser73 (via the MAPK pathway), it associates with co-activators of the p300/CBP family and enhances transcription. MITF has several isoforms including MITF-M which is specifically expressed in melanocytes. In MITF-deficient mice there is a complete absence of melanocytes. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 55 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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