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Anti-MID1IP1 Antibody (2E149)

😃Good
Catalog No. TMAY-02677
Alias THRSPL, STRAIT11499, S14R, MIG12, MID1 interacting protein 1, G12-like

Anti-MID1IP1 Antibody (2E149) is a Rabbit antibody targeting MID1IP1. Anti-MID1IP1 Antibody (2E149) can be used in WB,ICC/IF.

Anti-MID1IP1 Antibody (2E149)

Anti-MID1IP1 Antibody (2E149)

😃Good
Catalog No. TMAY-02677Alias THRSPL, STRAIT11499, S14R, MIG12, MID1 interacting protein 1, G12-like
Anti-MID1IP1 Antibody (2E149) is a Rabbit antibody targeting MID1IP1. Anti-MID1IP1 Antibody (2E149) can be used in WB,ICC/IF.
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100 μL$269 7-10 days
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Product Introduction

Bioactivity
Description
Anti-MID1IP1 Antibody (2E149) is a Rabbit antibody targeting MID1IP1. Anti-MID1IP1 Antibody (2E149) can be used in WB,ICC/IF.
AliasTHRSPL, STRAIT11499, S14R, MIG12, MID1 interacting protein 1, G12-like
Ig Type
Monoclonal Rabbit IgG
Clone
2E149
Reactivity
Human
Verified Activity
1. Anti-MID1IP1 rabbit monoclonal antibody at 1:500 dilution.

-Lane A: HepG2 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:20 kDa.

-Observed band size:20 kDa.

2. Immunofluorescence staining of MID1IP1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human MID1IP1 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoskeleton.
Application
WB,ICC/IF
Recommended Dose
WB: 1:500-1:1000; ICC-IF: 1:20-1:100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human MID1IP1 (rh MID1IP1; TMPY-03495; Q9NPA3; Met1-His183).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundMID1IP1 (MID1 Interacting Protein 1) is a Protein Coding gene. The encoded protein belongs to the SPOT14 family. It is a homodimer in the absence of THRSP. MID1IP1 interacts with ACACA and ACACB. Its interaction with THRSP interferes with ACACA binding. It up-regulates ACACA enzyme activity and plays a role in the regulation of lipogenesis in the liver. MID1IP1 is required for efficient lipid biosynthesis, including triacylglycerol, diacylglycerol, and phospholipid. MID1IP1 is involved in the stabilization of microtubules. It is widely expressed in bone marrow, fat, and other tissues. Diseases associated with MID1IP1 include Gluten Allergy and Scoliosis. Among its related pathways are the Import of palmitoyl-CoA into the mitochondrial matrix and Metabolism.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human MID1IP1 Protein (TMPY-03495)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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