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Anti-MID1IP1 Antibody (2E149)
😃Good
Catalog No. TMAY-02677
Alias THRSPL, STRAIT11499, S14R, MIG12, MID1 interacting protein 1, G12-like
Anti-MID1IP1 Antibody (2E149) is a Rabbit antibody targeting MID1IP1. Anti-MID1IP1 Antibody (2E149) can be used in WB,ICC/IF.
Anti-MID1IP1 Antibody (2E149)
Anti-MID1IP1 Antibody (2E149)
😃Good
Catalog No. TMAY-02677Alias THRSPL, STRAIT11499, S14R, MIG12, MID1 interacting protein 1, G12-like
Anti-MID1IP1 Antibody (2E149) is a Rabbit antibody targeting MID1IP1. Anti-MID1IP1 Antibody (2E149) can be used in WB,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $269 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: WB, ICC/IF Reactivity: Human |
| Alias | THRSPL, STRAIT11499, S14R, MIG12, MID1 interacting protein 1, G12-like |
| Ig Type | Rabbit IgG |
| Clone | 2E149 |
| Reactivity | Human |
| Verified Activity | 1. Anti-MID1IP1 rabbit monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:20 kDa. -Observed band size:20 kDa. 2. Immunofluorescence staining of MID1IP1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human MID1IP1 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoskeleton. |
| Application | WB,ICC/IF |
| Recommended Dose | WB: 1:500-1:1000; ICC-IF: 1:20-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human MID1IP1 (rh MID1IP1; TMPY-03495; Q9NPA3; Met1-His183). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | MID1IP1 (MID1 Interacting Protein 1) is a Protein Coding gene. The encoded protein belongs to the SPOT14 family. It is a homodimer in the absence of THRSP. MID1IP1 interacts with ACACA and ACACB. Its interaction with THRSP interferes with ACACA binding. It up-regulates ACACA enzyme activity and plays a role in the regulation of lipogenesis in the liver. MID1IP1 is required for efficient lipid biosynthesis, including triacylglycerol, diacylglycerol, and phospholipid. MID1IP1 is involved in the stabilization of microtubules. It is widely expressed in bone marrow, fat, and other tissues. Diseases associated with MID1IP1 include Gluten Allergy and Scoliosis. Among its related pathways are the Import of palmitoyl-CoA into the mitochondrial matrix and Metabolism. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human MID1IP1 Protein (TMPY-03495) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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