Antibody Type: Rabbit Polyclonal

Application: WB,IHC-P,IHC-Fr,IF

Reactivity: Human,Mouse (predicted:Rat)

IgG

Human,Mouse (predicted:Rat)

1. Protein: mouse lung lysates at 30 μg;
Primary: Anti-MHC Class II (TMAB-01135) at 1:200;
Secondary: HRP conjugated Goat Anti-Rabbit Igg (secondary antibody) at 1: 3000;
ECL excitated the fluorescence;
Predicted band size: 27 kDa
Observed band size: 30 kDa
2. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-MHC Class II Polyclonal Antibody, Unconjugated (TMAB-01135) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample:
Lane 1: Lung (Mouse) Lysate at 40 μg
Lane 2: Cerebrum (Mouse) Lysate at 40 μg
Lane 3: LympHnode (Mouse) Lysate at 40 μg
Lane 4: Spleen (Mouse) Lysate at 40 μg
Primary: Anti-MHC Class II (TMAB-01135) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29/50 kDa
Observed band size: 50 kDa

WB,IHC-P,IHC-Fr,IF

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human HLA class II histocompatibility antigen DM beta chain

Human

3109

Class II