Anti-MFN1 Antibody (5U87) is a Mouse antibody targeting MFN1. Anti-MFN1 Antibody (5U87) can be used in ICC/IF,IF,IHC-Fr,IHC-P,WB.

IgG2a/Kappa

5U87

Rat,Mouse,Human

1. Blocking buffer: 5% NFDM/TBST
Primary dilution: 1:1000
Primary incubation condition: 4°C overnight
Secondary: Goat Anti-Mouse IgG H&L (HRP)
Lysate: HepG2, PC-3, PC-12, EL4.IL-12
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 84 kDa
Observed MW: 84 kDa
2. Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (MFN1) Monoclonal Antibody, Unconjugated (TMAB-01130) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (MFN1) Monoclonal Antibody, Unconjugated (TMAB-01130) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (MFN1) Monoclonal Antibody, Unconjugated (TMAB-01130) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (MFN1) Monoclonal Antibody, Unconjugated (TMAB-01130) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (MFN1) Monoclonal Antibody, Unconjugated (TMAB-01130) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (MFN1) Monoclonal Antibody, Unconjugated (TMAB-01130) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (MFN1) Monoclonal Antibody, Unconjugated (TMAB-01130) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

WB: 1:200-1000; IHC-P: 1:200-100; IHC-Fr: 1:200-100; ICC/IF: 1:200-100; IF: 1:200-100

Monoclonal

MFN1

Mitofusin-1

Mitochondrial markers,Mitophagy fission and fusion,Parkinson's disease,GTP,Mitochondria

Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks.