Anti-MFN1 Antibody (5U87) is a Mouse antibody targeting MFN1. Anti-MFN1 Antibody (5U87) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG2a/Kappa

5U87

Rat,Mouse,Human

1. Blocking buffer: 5% NFDM/TBST
Primary dilution: 1:1000
Primary incubation condition: 4°C overnight
Secondary: Goat Anti-Mouse IgG H&L (HRP)
Lysate: HepG2, PC-3, PC-12, EL4.IL-12
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 84 kDa
Observed MW: 84 kDa
2. Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (MFN1) Monoclonal Antibody, Unconjugated (TMAB-01130) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (MFN1) Monoclonal Antibody, Unconjugated (TMAB-01130) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (MFN1) Monoclonal Antibody, Unconjugated (TMAB-01130) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (MFN1) Monoclonal Antibody, Unconjugated (TMAB-01130) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (MFN1) Monoclonal Antibody, Unconjugated (TMAB-01130) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (MFN1) Monoclonal Antibody, Unconjugated (TMAB-01130) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (MFN1) Monoclonal Antibody, Unconjugated (TMAB-01130) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

WB,IHC-P,IHC-Fr,ICC/IF,IF

WB: 1:200-1000; IHC-P: 1:200-100; IHC-Fr: 1:200-100; ICC/IF: 1:200-100; IF: 1:200-100

Monoclonal

Mitochondrial markers,Mitophagy fission and fusion,Parkinson's disease,GTP,Mitochondria