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Anti-METTL3 Polyclonal Antibody
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Catalog No. TMAB-01127
Alias N(6)-adenosine-methyltransferase catalytic subunit METTL3, N(6)-adenosine-methyltransferase 70 kDa subunit (MT-A70), MTA70, METTL3, Methyltransferase-like protein 3 (hMETTL3)
Anti-METTL3 Polyclonal Antibody is a Rabbit antibody targeting METTL3. Anti-METTL3 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P.
Anti-METTL3 Polyclonal Antibody
Anti-METTL3 Polyclonal Antibody
😃Good
Catalog No. TMAB-01127Alias N(6)-adenosine-methyltransferase catalytic subunit METTL3, N(6)-adenosine-methyltransferase 70 kDa subunit (MT-A70), MTA70, METTL3, Methyltransferase-like protein 3 (hMETTL3)
Anti-METTL3 Polyclonal Antibody is a Rabbit antibody targeting METTL3. Anti-METTL3 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-METTL3 Polyclonal Antibody is a Rabbit antibody targeting METTL3. Anti-METTL3 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P. |
| Synonyms | N(6)-adenosine-methyltransferase catalytic subunit METTL3, N(6)-adenosine-methyltransferase 70 kDa subunit (MT-A70), MTA70, METTL3, Methyltransferase-like protein 3 (hMETTL3) |
| Ig Type | IgG |
| Reactivity | Rat (predicted:Human,Mouse,Pig,Cow,Rabbit,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (METTL3) Polyclonal Antibody, Unconjugated (TMAB-01127) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (METTL3) Polyclonal Antibody, Unconjugated (TMAB-01127) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.   |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus speckle. Colocalizes with speckles in interphase nuclei. Suggesting that it may be associated with nuclear pre-mRNA splicing components. |
| Tissue Specificity | Widely expressed at low level. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthetic peptide: human METTL3 |
| Antigen Species | Human |
| Gene Name | METTL3 |
| Gene ID | |
| Protein Name | N6-adenosine-methyltransferase catalytic subunit |
| Uniprot ID | |
| Biology Area | DNA / Nucleotides,RNA Processing |
| Function | N6-methyltransferase that methylates adenosine residues of some mRNAs. N6-methyladenosine (m6A), which is present at internal sites of some mRNAs, may play a role in the efficiency of mRNA splicing, transport or translation. |
| Molecular Weight | Theoretical: 64 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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