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Anti-METAP2 Antibody (1F427)
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Catalog No. TMAY-03340
Alias p67eIF2, p67, Mnpep, methionyl aminopeptidase 2, AU014659, Amp2, AL024412, AI047573, A930035J23Rik, 4930584B20Rik
Anti-METAP2 Antibody (1F427) is a Rabbit antibody targeting METAP2. Anti-METAP2 Antibody (1F427) can be used in WB,IP.
Anti-METAP2 Antibody (1F427)
Anti-METAP2 Antibody (1F427)
😃Good
Catalog No. TMAY-03340Alias p67eIF2, p67, Mnpep, methionyl aminopeptidase 2, AU014659, Amp2, AL024412, AI047573, A930035J23Rik, 4930584B20Rik
Anti-METAP2 Antibody (1F427) is a Rabbit antibody targeting METAP2. Anti-METAP2 Antibody (1F427) can be used in WB,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $268 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-METAP2 Antibody (1F427) is a Rabbit antibody targeting METAP2. Anti-METAP2 Antibody (1F427) can be used in WB,IP. |
| Alias | p67eIF2, p67, Mnpep, methionyl aminopeptidase 2, AU014659, Amp2, AL024412, AI047573, A930035J23Rik, 4930584B20Rik |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 1F427 |
| Reactivity | Mouse |
| Verified Activity | 1. Anti-METAP2 rabbit monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:52 kDa. -Observed band size:65 kDa. 2. Mouse METAP2 was immunoprecipitated using: -Lane A:0.5 mg HepG2 Whole Cell Lysate. -2 µL anti-Mouse METAP2 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-Mouse METAP2 rabbit monoclonal antibody, at 1:200 dilution. -Secondary antibody: -Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution. -Developed using the DAB staining technique. -Performed under reducing conditions. -Predicted band size: 52 kDa. -Observed band size: 72 kDa |
| Application | WB,IP |
| Recommended Dose | WB: 1:500-1:2000; IP: 0.5-2 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse MAP2 / METAP2 (rM MAP2 / METAP2; TMPY-02727; O08663; Ala2-Tyr478). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | METAP2 (Methionine aminopeptidase 2), also known as MAP2 is a protein that belongs to the peptidase M24A family. MAP2 binds 2 cobalt or manganese ions and contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. It is found in all organisms and is especially important because of its critical role in tissue repair and protein degradation. The catalytic activity of human MAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. MAP2 protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. It also plays a critical role in the regulation of protein synthesis. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Mouse MAP2 / METAP2 protein (TMPY-02727) |
| Antigen Species | Mouse |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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