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Anti-MetAP1 Polyclonal Antibody
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Catalog No. TMAB-08764
Anti-MetAP1 Polyclonal Antibody is a Rabbit antibody targeting MetAP1. Anti-MetAP1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-MetAP1 Polyclonal Antibody
Anti-MetAP1 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-08764
Anti-MetAP1 Polyclonal Antibody is a Rabbit antibody targeting MetAP1. Anti-MetAP1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Antigen Details
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| Description | Anti-MetAP1 Polyclonal Antibody is a Rabbit antibody targeting MetAP1. Anti-MetAP1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Chicken,Dog,Cow,Horse,Rabbit) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MetAP1) Polyclonal Antibody, Unconjugated (TMAB-08764) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MetAP1) Polyclonal Antibody, Unconjugated (TMAB-08764) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | MetAP-1 is a 394-amino acid protein that is expressed at low levels in all tissues, but is highly expressed in skeletal muscles. The active site of MetAP-1 contains two adjacent divalent metal ions connected by a water molecule or hydroxide ion. The control of cell proliferation in mammalian cells is directly linked and strictly dependent on the evolutionarily highly conserved mechanism that MetAP-1 employs. Eukaryotes contain both MetAP-1 and MetAP-2, whereas prokaryotes possess only the MetAP-1 enzyme. Pyridine-2-carboxylic acid thiazol-2-ylamide (PCAT) forms a scaffold that inhibits the action of MetAP-1, while 1,2,4-triazol is a non-peptide inhibitor of MetAP-1 binding to the active site with the N1 and N2 atoms of the triazole moiety complexing two divalent ions. |
| Immunogen | KLH conjugated synthetic peptide: human MetAP1 |
| Antigen Species | Human |
| Gene Name | METAP1 |
| Gene ID | |
| Protein Name | Methionine aminopeptidase 1 |
| Uniprot ID | |
| Function | Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Required for normal progression through the cell cycle. |
| Molecular Weight | Theoretical: 43 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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