Anti-MEPCE Polyclonal Antibody

Anti-MEPCE Polyclonal Antibody is a Rabbit antibody targeting MEPCE. Anti-MEPCE Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM,ICC/IF.

IgG

Human,Mouse (predicted:Rat,Dog,Pig,Cow,Rabbit,Sheep,Rhesus monkey)

1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MEPCE) Polyclonal Antibody, Unconjugated (TMAB-08751) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. 4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (MEPCE) polyclonal Antibody, unconjugated (TMAB-08751) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
3. The MCF-7 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Rabbit Anti-MEPCE antibody (TMAB-08751): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test; ICC/IF: 1:50-200

Polyclonal

KLH conjugated synthetic peptide: human MEPCE

Human

MEPCE

7SK snRNA methylphosphate capping enzyme

S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, leading to stabilize it.