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Anti-MEKK2 Antibody (5G332)
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Catalog No. TMAC-02556
Alias Mitogen-activated protein kinase kinase kinase 2, Mitogen activated protein kinase kinase kinase 2, MEKK2b, MEKK2, MEK kinase 2, MAPK/ERK kinase kinase 2, Map3k2, M3K2_HUMAN
Anti-MEKK2 Antibody (5G332) is a Rabbit antibody targeting MEKK2. Anti-MEKK2 Antibody (5G332) can be used in WB,ICC,IHC,IP,FCM.
Anti-MEKK2 Antibody (5G332)
Anti-MEKK2 Antibody (5G332)
😃Good
Catalog No. TMAC-02556Alias Mitogen-activated protein kinase kinase kinase 2, Mitogen activated protein kinase kinase kinase 2, MEKK2b, MEKK2, MEK kinase 2, MAPK/ERK kinase kinase 2, Map3k2, M3K2_HUMAN
Anti-MEKK2 Antibody (5G332) is a Rabbit antibody targeting MEKK2. Anti-MEKK2 Antibody (5G332) can be used in WB,ICC,IHC,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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| Description | Anti-MEKK2 Antibody (5G332) is a Rabbit antibody targeting MEKK2. Anti-MEKK2 Antibody (5G332) can be used in WB,ICC,IHC,IP,FCM. |
| Synonyms | Mitogen-activated protein kinase kinase kinase 2, Mitogen activated protein kinase kinase kinase 2, MEKK2b, MEKK2, MEK kinase 2, MAPK/ERK kinase kinase 2, Map3k2, M3K2_HUMAN |
| Ig Type | IgG |
| Clone | 5G332 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of MEKK2 on different lysates using anti-MEKK2 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2, Lane 2: Rat brain, Lane 3: SW480. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MEKK2 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MEKK2 antibody. Counter stained with hematoxylin. 4. ICC staining MEKK2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining MEKK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining MEKK2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of HepG2 cells with MEKK2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli including growth factors. The MEK kinases (also designated MAP kinase kinase kinases, MKKKs, MAP3Ks or MEKKs) phosphorylate and thereby activate the MEKs (also called MAP kinase kinases or MKKs), including ERK, JNK and p38. These activated MEKs in turn phosphorylate and activate the MAP kinases. The MEK kinases include Raf-1, Raf-B, Mos, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4, ASK 1 (MEK kinase-5) and MAP3K6 (MEK kinase-6). MEK kinase-1 has been shown to phosphorylate MEK-1 via a Raf-independent pathway. Evidence suggests that MEK-3 is preferentially activated by MEK kinase-3 and that MEK-4 is activated by both MEK kinase-2 and MEK kinase-3. MEK kinase-4 has been shown to specifically activate the JNK pathway. ASK 1 activates both MEK-4 and MEK-3/MEK-6 pathways. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 70 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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