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Anti-MEK3 Polyclonal Antibody
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Catalog No. TMAB-08734
Anti-MEK3 Polyclonal Antibody is a Rabbit antibody targeting MEK3. Anti-MEK3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-MEK3 Polyclonal Antibody
Anti-MEK3 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-08734
Anti-MEK3 Polyclonal Antibody is a Rabbit antibody targeting MEK3. Anti-MEK3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-MEK3 Polyclonal Antibody is a Rabbit antibody targeting MEK3. Anti-MEK3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Sample: Muscle (Mouse) Lysate at 40 μg Muscle (Mouse) Lysate at 40 μg Primary: Anti-MEK3 (TMAB-08734) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kD Observed band size: 39 kD 2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MEK3) Polyclonal Antibody, Unconjugated (TMAB-08734) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MEK3) Polyclonal Antibody, Unconjugated (TMAB-08734) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 4. Formalin-fixed and paraffin embedded human skeletal muscle labeled with Rabbit Anti-MEK3/MAP2 k3 Polyclonal Antibody (TMAB-08734) at 1: 250 overnight at room temperature followed by conjugation to secondary antibody. 5. Sample: Skeletal muscle (Rat) Lysate at 40 μg Primary: Anti-MEK3 (TMAB-08734) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kD Observed band size: 39 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm; cytoskeleton. |
| Tissue Specificity | Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq]. |
| Immunogen | KLH conjugated synthetic peptide: human MKK3 |
| Antigen Species | Human |
| Gene Name | MAP2K3 |
| Gene ID | |
| Protein Name | Dual specificity mitogen-activated protein kinase kinase 3 |
| Uniprot ID | |
| Function | Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. |
| Molecular Weight | Theoretical: 39 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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